نتایج جستجو برای: kanr
تعداد نتایج: 38 فیلتر نتایج به سال:
BACKGROUND One problem with engineered genetic circuits in synthetic microbes is their stability over evolutionary time in the absence of selective pressure. Since design of a selective environment for maintaining function of a circuit will be unique to every circuit, general design principles are needed for engineering evolutionary robust circuits that permit the long-term study or applied use...
BACKGROUND In the presence of sufficient iron, the Escherichia coli protein Fur (Ferric Uptake Regulator) represses genes controlled by the Fur box, a consensus sequence near or within promoters of target genes. De-repression of Fur-controlled genes occurs upon iron deprivation. In the E. coli chromosome, there is a bidirectional intercistronic promoter region with two non-overlapping Fur boxes...
We determined sites in X ell mRNA that are cleaved by RNase III in the presence of X OOP antisense RNA, using a series of OOP RNAs with different internal deletions. In OOP RNA-cll mRNA structures containing a potential region of continuous double-stranded RNA bounded by a non-complementary unpaired region, RNase III cleaved the ell mRNA at one or more preferred sites located 10 to 14 bases fro...
Rapamycin is a potent immunosuppressant that blocks the G1/S transition in antigen-activated T cells and in yeast. The similar effects of rapamycin in animal cells and yeast suggest that the biochemical steps affected by rapamycin are conserved. Using a two-hybrid system we isolated mammalian clones that interact with the human FK506/rapamycinbinding protein (FKBP12) in the presence of rapamyci...
Article26 April 2021Open Access Source DataTransparent process Single-cell dynamics of chromatin activity during cell lineage differentiation in Caenorhabditis elegans embryos Zhiguang Zhao orcid.org/0000-0002-0084-3783 State Key Laboratory Molecular Developmental Biology, Institute Genetics and Chinese Academy Sciences, Beijing, China University ChinaThese authors contributed equally to this w...
We show that temperature-sensitive mutations in dnaZ, the gene for the y subunit of DNA polymerase 111 holoenzyme, can be suppressed by mutations in the dnaN gene, which encodes the P subunit. These results support a direct physical interaction of these two subunits during polymerase assembly or function. The suppressor phenotype is also sensitive to modulation by the dnaA genotype. Since dnaA ...
The MSHl and MSH2 genes of Saccharomyces cerevisiae are predicted to encode proteins that are homologous to the Escherichia coli MutS and Streptococcus pneumoniae HexA proteins and their homologs. Disruption of the MSHl gene caused a petite phenotype which was established rapidly. A functional MSHl gene present on a single-copy centromere plasmid was incapable of rescuing the established mshl p...
Die fermentative Ganzzellproduktion in Escherichia coli ermöglicht die Produktion komplexer Naturstoffe, wie Terpene, aus einfachen, günstigen und nachhaltigen C-Quellen. Ziel der Arbeit war es, einen solchen Prozess zur Synthese von Presilphiperfolan-8β-ol (PSP) zu entwickeln. Der Biosyntheseweg dieses tricyclischen Sesquiterpenalkohols konnte erfolgreich E. eingebracht werden. resultierende M...
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