A large number of hprt-mutants were obtained by treating human lymphoblast cells (TK6) with 5 microM K2Cr2O7 for 5 hr and selecting by growth in 6-thioguanine. A combination of high fidelity polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE) allowed us to measure mutant frequencies as a function of DNA sequence. Chromium(VI) induced four hotspots in a 104 bp doma...

A tubular gas diffusion PTFE membrane is exploited for non-invasive sampling in flow analysis, aiming to develop an improved spectrophotometric determination of ethanol in alcoholic beverages. The probe is immersed into the sample, allowing ethanol to diffuse through the membrane. It is collected into the acceptor stream (acidic dichromate solution), leading to formation of Cr(III), monitored a...

Evolution of Liesegang patterns in an electric field was studied experimentally in the AgNO3/K2Cr2O7/gelatine system. The distance of the last (nth) band as a function of their appearance time can be described by the equation Xn 1⁄4 c1t+ c2t+ c3 . A numerical model, based on Ostwald ’s supersaturation theory, predicted the same functional law. Experiments showed that the ratio of the distances ...

Investigation and development of a separating mesa-structure creating technology for fabricating multi-junction solar cells based on the GaInP/GaInAs/Ge heterostructure has been carried out. Studied were methods etching layers: liquid chemical in etchants HBr, H2O2, K2Cr2O7 plasma-chemical stream operating gas BCl3. The comparative analysis was studied. protective masks photoresist layer TiOx/S...

This experiment was carried out to evaluate the effect of specific gravity (SG) and particle size (PS) of Chromium-mordanated alfalfa neutral detergent fiber (NDF) markers on estimation of particulate rumen turnover. Thirty-two multiparous, mid lactation Holstein dairy cows (body weight=654±24 kg) were allotted to a completely randomized design experiment with two replications in a 4×4 factoria...

A flow injection method (FI) for the sequential determination of ethanol and acetic acid in vinegar is reported. The determination of ethanol is based on the oxidation of the pervaporated ethanol by K2Cr2O7. The acetic acid is determined by an acid-base reaction with Thymol Blue as the indicator. Both reactions are monitored photometrically at 600 nm using a single detector. Optimisation studie...