Molecular genetic fingerprints of eleven Hedychium species from Northeast India were developed using PCR based markers. Fifteen inter-simple sequence repeats (ISSRs) and five amplified fragment length polymorphism (AFLP) primers produced 547 polymorphic fragments. Positive correlation (r = 0.46) was observed between the mean genetic similarity and genetic diversity parameters at the inter-speci...

Se realizó una estandarización del método de extracción ADN para cañihua (Chenopodium pallidicaule Aellen) y la caracterización molecular mediante marcadores moleculares ISSR (Inter Simple Sequence Repeat). utilizaron hojas 40 accesiones tomadas banco germoplasma Programa Cereales Granos Nativos UNALM provenientes dos provincias Puno. el CTAB. La agregación cloroformo: alcohol isoamílico (24:1)...

In this study, molecular characterization of Dittrichia viscosa (L.) Greuter populations collected from Ayd?n province was carried out based on ISSR technique and chloroplast DNA trnL intron sequences. 10 primers were used to determine the among populations. For cpDNA amplification, trnC trnD used. analysis, a total 70 bands obtained. The polymorphism rate determined be approximately 94.28%. Ac...

In the present study, 20 sandalwood (Santalum album L.) genotypes were characterized using RAPD, ISSR and SSR markers. Twenty-five RAPD and twenty-one ISSR primers that generated clear and reproducible banding patterns amplified 225 and 208 bands, respectively, among 20 sandalwood genotypes. Out of 225, 181 (83.13 %) RAPD bands were polymorphic while out of 208, 156 (75.77 %) ISSR bands were po...

Brycon is one of the main genera of Neotropical freshwater fish. In Brazil, Brycon species have been found in many hydrographic basins, such as the Amazon, Paraná, Paraguay, and Araguaia-Tocantins basins. We examined the phylogenetic relationships among the species Brycon orbignyanus, B. hilarii, B. cf. pesu, B. cephalus, B. falcatus, and B. gouldingi, using mitochondrial and nuclear molecular ...

Sequence-related amplified polymorphism (SRAP) and inter-simple sequence repeat (ISSR) markers were used to assess the genetic diversity within and among 15 natural populations of Stipa bungeana from the Loess Plateau of China. Using 15 SRAP primers, 504 (99.80%) polymorphic loci were detected, and 372 polymorphic loci (96.12%) were identified using 15 ISSR primers. At the species level, the S....

Background: The prevailing methods for the genomic DNA extraction of Sclerotium rolfsii from its mycelium mat is often time consuming and yields poor quality quantity owing to presence outrageous magnitude mucilage polysaccharides. Methods: A new fast track method isolation resting structure (sclerotia) S. by modifying CTAB deprived supplementation proteinase K has been standardized. Result: pr...

The rapid development of DNA sequencing technology in recent years has provided new tools for phylogenetic data acquisition. By using high-throughput technology, molecular information can be obtained more quickly and economically. Here, we describe a complementary combination two multiplex methods. One is multiplexed marker (MPM-seq), the other inter-simple sequence repeat (ISSR) genotyping by ...

Erythropalum scandens Blume, an emerging medicinal plant with great potential for drug development, also possesses high edible value. In this study, we investigated the genetic diversity of germplasm E. obtained from different geographical locations using inter simple sequence repeat (ISSR) markers. For purpose, 18 ISSR primer pairs a distinct background and adequate polymorphism were selected....

Kumar A, Verma N. 2012. A comparative phylogenetic analysis of medicinal plant Tribulus terrestris in Northwest India revealed by RAPD and ISSR markers. Biodiversitas 13: 107-113. Several DNA marker systems and associated techniques are available today for fingerprinting of plant varieties. A total of 5 RAPD and 8 ISSR primers were used. Amplification of genomic DNA of the 6 genotypes, using RA...