IMPORTANT NOTE: The smallest amount of contamination of DNA preparations by recombinant phages or plasmids can be disastrous. Many person-years have been wasted reisolating previously cloned sequences that contaminated preparations of DNA used to create recombinant DNA libraries and many researchers have been embarrassed to find that the “extra” genes they found on their Southern blots were act...

lipopolysaccharide (lps) is an important structural component of the outer cell membrane complex of gram-negative microorganisms. its causative role in gram-negative bacteria-induced diseases and broad applications in different kinds of cell stimulation experiments provided a conceptual basis for studies directed at the isolation, purification, and detailed chemical characterization of lps. the...

Recombinant protein purification is a kind of sensitive and expensive method in genetics engineering. Genetic manipulation leads to the expression of various proteins; it should be isolated with high purity finally. Differed methods for protein purification are categorized, based on cast, quality, Easy work and side effect of protein. In this article, we are investigating his His-tag protein pu...

An efficient and environmental-friendly synthetic route to 1,8-dioxodecahydroacridines derivatives have been developed via multi-component one-pot Hantzsch reaction of various aldehydes and ammonium acetate with 2 equivalents dimedone in the presence of Phosphorus pentoxide supported on aluminaas catalyst under solvent-free conditions. The present approach offersseveral advantages such as short...

An efficient and environmental-friendly synthetic route to 1,8-dioxodecahydroacridines derivatives have been developed via multi-component one-pot Hantzsch reaction of various aldehydes and ammonium acetate with 2 equivalents dimedone in the presence of Phosphorus pentoxide supported on aluminaas catalyst under solvent-free conditions. The present approach offersseveral advantages such as s...

An efficient and environmental-friendly synthetic route to 1,8-dioxodecahydroacridines derivatives have been developed via multi-component one-pot Hantzsch reaction of various aldehydes and ammonium acetate with 2 equivalents dimedone in the presence of Phosphorus pentoxide supported on aluminaas catalyst under solvent-free conditions. The present approach offersseveral advantages such as s...