In light of the multitude of new Cas9-mediated functionalities, the ability to carry out multiple Cas9-enabled processes simultaneously and in a single cell is becoming increasingly valuable. Accomplishing this aim requires a set of Cas9-guide RNA (gRNA) pairings that are functionally independent and insulated from one another. For instance, two such protein-gRNA complexes would allow for concu...

We present a principled approach to selecting a surface reflectance model and setting its parameters. The approach is based on fundamental physical principles, comparisons between different models, and practical insight into the characteristics of real surfaces. Four models of surface reflectance were evaluated by comparing them with physical measurements on five different sample surfaces. Conc...

Regulatory Guides are issued to describe and make available to the public such information as methods acceptable to the NRC staff for implementing specific parts of the Commission’s regulations, techniques used by the staff in evaluating specific problems or postulated accidents, and data needed by the NRC staff in its review of applications for permits and licenses. Regulatory Guides are not s...

Regulatory guides are issued to describe and make available to the public such information as methods acceptable to the NRC staff for implementing specific parts of the NRC’s regulations, techniques used by the staff in evaluating specific problems or postulated accidents, and data needed by the NRC staff in its review of applications for permits and licenses. Regulatory guides are not substitu...

peripheral nerve injuries (pni) can lead to lifetime loss of function and disfigurement. different methods such as conventional allograft procedures and using of biological tubes have problems for damaged peripheral nerves reconstruction. designed scaffolds with natural and synthetic materials are now widely used in the reconstruction of damaged tissues. utilization of absorbable and non-absorb...

conclusions we found the magnetic field assistant device to be an accurate, radiation-independent jig for close passing of guide wire after close reduction of femoral fracture. it can reduce the need for radiation during placement of guide wire for closed intramedullary nailing of the long bones. more studies are required to improve and evaluate the technique and equipment. results outcomes of ...

at the final stages of a coronary artery bypass graft operation on a 64-year-old man, an experienced physician attempted to insert an intra-aortic balloon pump into the femoral artery via the seldinger technique. however, while the balloon pump was being passed over the guide wire, the latter was completely lost. the guide wire should be held at the tip at all times to prevent passage into the ...

A new method (thermal spokes) is proposed to simulate the guide rolls in FE analysis of the ring rolling process. So far this method is the only one, capable of calculating guide rolls reaction contact forces related to the stiffness of their adjustment mechanism. The method is simple to use, does not introduce further nonlinearities and could be used in any kind of FE formulations. The method ...

A new method (thermal spokes) is proposed to simulate the guide rolls in FE analysis of the ring rolling process. So far this method is the only one, capable of calculating guide rolls reaction contact forces related to the stiffness of their adjustment mechanism. The method is simple to use, does not introduce further nonlinearities and could be used in any kind of FE formulations. The method ...