Iwamoto, Takahiro. Vascular Na /Ca exchanger: implications for the pathogenesis and therapy of salt-dependent hypertension. Am J Physiol Regul Integr Comp Physiol 290: R536–R545, 2006; doi:10.1152/ajpregu.00592.2005.—The Na /Ca exchanger is an ion transporter that exchanges Na and Ca in either Ca efflux or Ca influx mode, depending on membrane potential and transmembrane ion gradients. In arter...

The task of purifying monoclonal antibodies (MAbs) and human recombinant antithrombin III (rATIII) from cell culture supernatant was carried out using two different approaches, both based on the use of membraneous matrices. The first approach employed a strongly acidic and a strongly basic membrane ion exchanger, which were evaluated for their ability to purify monoclonal antibodies and the hum...

Iwamoto, Takahiro. Vascular Na /Ca exchanger: implications for the pathogenesis and therapy of salt-dependent hypertension. Am J Physiol Regul Integr Comp Physiol 290: R536–R545, 2006; doi:10.1152/ajpregu.00592.2005.—The Na /Ca exchanger is an ion transporter that exchanges Na and Ca in either Ca efflux or Ca influx mode, depending on membrane potential and transmembrane ion gradients. In arter...

Yeast Nhx1 [Na+(K+)/H+ exchanger 1] is an intracellular Na+(K+)/H+ exchanger, localizing to the late endosome where it is important for ion homoeostasis and vesicle trafficking. Phylogenetic analysis of NHE (Na+/H+ exchanger) sequences has identified orthologous proteins, including HsNHE6 (human NHE6), HsNHE7 and HsNHE9 of unknown physiological role. These appear distinct from well-studied mamm...

The membrane-bound sodium-calcium exchanger (NCX) proteins shape Ca2+ homeostasis in many cell types, thus participating in a wide range of physiological and pathological processes. Determination of the crystal structure of an archaeal NCX (NCX_Mj) paved the way for a thorough and systematic investigation of ion transport mechanisms in NCX proteins. Here, we review the data gathered from the X-...

Ion-sensitive microelectrodes and current-voltage analysis were used to study intracellular pH (pHi) regulation and its effects on ionic conductances in the isolated epithelium of frog skin. We show that pHi recovery after an acid load is dependent on the operation of an amiloride-sensitive Na+/H+ exchanger localized at the basolateral cell membranes. The antiporter is not quiescent at physiolo...

To investigate the relationship between 12 candidate genes responsible for water regulation, sodium metabolism and membrane ion transport and essential hypertension (EH) in the Chinese. Linkage analysis of EH was performed in 95 Chinese nuclear families including 477 subjects using a technique of fluorescence-based gene scanning with 12 microsatellite markers. Markers were selected on the chrom...

The aim of the work was to elucidate the nature of charge-selective properties of macroporous composite inorganic membranes modified with nanoparticles of hydrated zirconium dioxide. The membranes have been investigated using methods of standard contact porosimetry, potentiometry, electron microscopy and small-angle X-ray scattering. The ion exchanger has been found to deposit inside pores of c...