Gold nanoparticles have been available for many years as a research tool in the life sciences due to their electron density and optical properties. New applications are continually being developed, particularly in nanomedicine. One drawback is the need for an easy, real-time quantitation method for gold nanoparticles so that the effects observed in in vitro cell toxicity assays and cell uptake ...

1. A compound of hyaluronate and protein, called hyaluronate-protein was isolated from pooled human synovial fluids by caesium chloride density-gradient ultracentrifugation. 2. The isolated hyaluronate-protein was labelled with [(125)I]iodide and the following studies were done. (a) Ultracentrifugation in caesium chloride showed that the protein moiety ((125)I counts) and hyaluronate (hexuronat...

A dose-response study was performed in adult rats to select an optimal stable potassium iodide (KI) dose which could be implemented in repeated prophylaxis, in case of prolonged exposure to radioactive iodine. Increasing doses of KI were given orally to rats 1 hour before internal exposure simulated by I-125 injection. I-125 incorporation in the thyroid was measured by γ-spectrometry, and KI pr...

reduction of methoxyketon 1 by nabh4 gives methoxyketol 2. allthough acetate and tosylate of this alchohol were not seprated, benzoate ester was isolated. reduction of ketol 2 by lialh4 gives alchohol 4. ketol 9 does not react with methyl magnesium iodide, but methoxyketon 1 react with both methyl magnesium iodide and phenyl magnesium iodide gives corresponding hydrocarbons. keton 13 with pheny...

Iodine-125 (125I) is one of the radioisotopes widely used in radiopharmaceuticals for diagnosis and therapy various cancers. Recent reports indicate that there has been shortages world supply this radioiodine isotope. One absolute requirements good must meet radiochemical purity, which generally to be above 95 %, with an efficiency over 90 %. The previous investigation shows purity low does not...

The cell cortex (or pellicle) of Tetrahymena is composed of three closely associated layers: the cell membrane, the alveolar sacs, and the epiplasm. The epiplasm is the innermost layer of the cortex and is thought to be analogous to the membrane skeleton found in eukaryotic cells, since it consists largely of filamentous proteins responsible for maintaining cell stability and shape. Furthermore...

Iodine has many important biological functions and its concentrations vary with the environment. Recent research has provided novel insights into iodine uptake mechanisms in marine bacteria and kelp through hydrogen peroxide-dependent diffusion (PDD). This mechanism is distinct from sodium-dependent mechanisms known from vertebrates. In vertebrates, iodine accumulates in the thyroid gland by th...

A procedure for the isolation and separation of three different subfractions of plasma membrane from the cellular slime mould Dictyostelium discoideum is described. The cells were disrupted by freeze-thawing in liquid N(2) and plasma membranes were purified by equilibrium centrifugation in a sucrose gradient. The cell surface was labelled with radioactive iodide by using the lactoperoxidase iod...

Adequate iodide supply and metabolism are essential for thyroid hormones synthesis. In thyrocytes, iodide uptake is mediated by the sodium-iodide symporter, but several proteins appear to be involved in iodide efflux. Previous studies demonstrated that pendrin is able to mediate apical efflux of iodide in thyrocytes. Acute iodide excess transiently impairs thyroid hormone synthesis, a phenomeno...