Previous studies from this laboratory have demonstrated the oxidation of certain proteins by tyrosinase (1, 2) and the inactivation of invertase by tyrosinase (3, 4). Different tyrosinase preparations vary greatly in their ability to inactivate invertase; in particular, certain dialyzed or very highly purified tyrosinase solutions seem to have lost their ability to oxidize invertase. In Paper I...

1. Invertase ist salzempfindlich nur in Gegenwart von Kolloiden, wie Gelatine, Albumin, Globulin, Lecithin; ihre Aktivität wird dabei durch die Salze herabgesetzt. 2. An den Komplexen Invertase-Globulin und Invertase-Lecithin lässt sich eine Ionenaequilibrierung von der Art des physiologischen Kationenantagonismus nachweisen; Na und K einerseits, Mg, Ca, Sr, Ba, Al, La andererseits hemmen in ge...

The increase in the invertase activity of extracts from freshly cut potato (Solanum tuberosum L.) by "foaming," caused by selective denaturation of an endogenous invertase inhibitor, did not occur in extracts made from thin disks 2 days after slicing. Rather, foaming such extracts decreased invertase activity. Apparently, the inhibitor disappeared after slicing, and the enzyme became more labil...

The effect of inositol supplementation on glucose derepression, invertase secretion and SUC2 gene expression in Saccharomyces sp. W4 was studied. Invertase secretion was repressed, when the yeast cells, grown the synthetic medium without inositol (I(-) medium) contained more than 0.2% (w/v) initial concentration of glucose. However, in the same medium plus inositol (I(+) medium, inositol conc. ...

The possible role of the sucrose-splitting enzymes sucrose synthase and invertase in elongating roots and hypocotyls of Arabidopsis was tested by using a combination of histochemical methods and quantitative trait locus (QTL) analysis. Lengths of roots and hypocotyls correlated better with invertase activities than with sucrose synthase activities. The highest correlations were observed with ac...

Saul and Nelson (1) studied the influence of proteins on the activity of yeast invertase, and found that native and denatured proteins accelerate the activity of purified invertase preparations at pH 3, but not at pH 4.5, the pH optimum of the enzyme. Herriott (2) had shown that yeast invertase inactivated by acid can be reactivated by following Anson and Mirsky's (3) procedure of allowing a so...

Nine mutations in the signal sequence region of the gene specifying the secreted Saccharomyces cerevisiae enzyme invertase were constructed in vitro. The consequences of these mutations were studied after returning the mutated genes to yeast cells. Short deletions and two extensive substitution mutations allowed normal expression and secretion of invertase. Other substitution mutations and long...

The activity of invertase and its relation to growth were studied in the epicotyls of lentil seedlings incubated in the presence and absence of gibberellic acid (GA(3)).Invertase activity per epicotyl increases relatively more rapidly than does length, reaches a maximum during most active elongation, and declines upon cessation of growth.GA(3) enhances both growth and increase in invertase acti...

Suc is the final product of photosynthesis, but before it can be utilized it must be cleaved into hexoses either by invertase (0-fructofuranosidase, EC 3.2.1.26) or by SUC synthase (EC 2.4.1.13). There are three recognized types of invertase present in plant cells: soluble neutra1 invertase, soluble acid invertase, and insoluble cell-wall acid invertase. Invertase genes encoding cell-wall and v...

Saccharum officinarum is one of the most cultivated hybrid varieties among the sugarcane varieties. In sugarcane plant sucrose is the major carbohydrate which can be stored and transported. Different physiological and biochemical studies on this crop report that invertase activity and sucrose concentration some how are key limiting step in the process of sucrose accumulation. Significant effort...