The goal of this short communication is to report the uncommon presence of intracellular amastigotes of Leishmania in peritoneal fluid of a dog with leishmaniasis from Alagoas State, Brazil. Physical examination of an adult male rottweiler suspected to be suffering of leishmaniasis revealed severe loss of weight, ascitis, splenomegaly, moderately enlarged lymph nodes, onychogryphosis, generaliz...

It has been well documented that low concentrations of ANG II (10(-11) to 10(-10) M) stimulate, whereas high concentrations of ANG II (10(-8) to 10(-5) M) inhibit Na(+) transport in proximal tubules of rat and rabbit kidneys. Measured ANG II concentration in proximal tubular fluid is in the nanomolar range. In the present study, we investigated the role of PKC, intracellular Ca(2+), and cAMP in...

Although the concept of tissue buffering was formulated early in the study of acid-base disturbances, attention in the past has been directed primarily towards the effects of these disturbances on blood and extracellular fluid. More recently the demonstration by Darrow and his co-workers (1-3) of exchanges of intracellular cations during clinical and experimental potassium deficiency stimulated...

We constructed an electrolyte balance sheet for 11 individual specimens and two pooled specimens of breast-cyst fluid. Na+ and K+ ranged in concentrations from those representative of extracellular fluids (147 and 9 mmol/L, respectively) to those of intracellular fluid or a high K+ secretion (23 and 168 mmol/L, respectively). Cl- concentrations ranged from 9 to 98 mmol/L (mean, 31), bicarbonate...

Splenectomized and adrenalectomized-splenectomized dogs, anesthetized with pentobarbital, were bled so that the role of cortisol in restitution of blood volume could be examined. Intact dogs and adrenalectomized dogs infused with cortisol at a high rate (17 mug/min) showed restoration of blood volume and plasma protein at 24 h, preceded by an early increase in plasma osmolality. Adrenalectomize...

PURPOSE Assessment of post-exercise changes in hydration with bioimpedance (BI) is complicated by physiological adaptations that affect resistance (R) and reactance (Xc) values. This study investigated exercise-induced changes in R and Xc, independently and in bioelectrical impedance vector analysis, when factors such as increased skin temperature and blood flow and surface electrolyte accumula...

To increase our current understanding of cellular processes, such as cell signaling and division, knowledge is needed about the spatial and temporal organization of the proteome at different organizational levels. These levels cover a wide range of length and time scales: from the atomic structures of macromolecules for inferring their molecular function, to the quantitative description of thei...

About 60% of the adult human body is fluid. The most of this fluid is inside the cells and called intracellular fluid (ICF) and one third is in the space out side the cells and called extracellular fluid (ECF) (1). Evaluation of the two components of ECF and ICF is important in various disease conditions. The standard method for this purpose in clinical practice is dilution principle using a tr...

Exhaustive 'burst-type' exercise in the rainbow trout resulted in a severe acidosis in the white muscle, with pHi dropping from 7.21 to a low of 6.62, as measured by DMO distribution. An accumulation of lactate and pyruvate, depletions of glycogen, ATP and CP stores, and a fluid shift from the extracellular fluid to the intracellular fluid of white muscle were associated with the acidosis. The ...