Colorectal cancers commonly carry truncation mutations in the adenomatous polyposis coli (APC) gene. The APC protein contributes to the stabilization of microtubules. Consistently, microtubules in cells lacking APC depolymerize more readily in response to microtubule-destabilizing drugs. This raises the possibility that such agents are suitable for treatment of APC-deficient cancers. However, A...

CENP-C, one of the few known intrinsic proteins of the human centromere, is thought to play structural as well as regulatory roles crucial to proper chromosome segregation and mitotic progression. To further define the functions of CENP-C throughout the cell cycle we have used the yeast interaction trap to identify proteins with which it interacts. One specific CENP-C interactor, which we have ...

Background: Exposure to ionizing radiation results in genotoxicity and the unrepaired lesions in cellular DNA results in cell cycle arrest, reproductive death, interphase death, division delay, chromosome aberrations, mutations, etc. leading to the intensive destruction of cells and violation of their proliferative capacity there by adversely affecting the mammalian system. Since ionizing radia...

Condensin complexes play vital roles in chromosome condensation during mitosis and meiosis. Condensin II uniquely localizes to chromatin throughout the cell cycle and, in addition to its mitotic duties, modulates chromosome organization and gene expression during interphase. Mitotic condensin activity is regulated by phosphorylation, but mechanisms that regulate condensin II during interphase a...

When two immiscible polymers are brought into intimate contact, highly localized mixing of polymer chains creates an “interphase” region. We have fabricated materials that are entirely interphase by forced assembly of two immiscible polymers. Assemblies of thousands of alternating layers of two polymers, with individual layer thickness on the nanometer size scale of the interphase, were created...

abstract background: to produce a reliable probe suitable for aneuploidy detection of chromosome 13 on uncultured lymphocytes and amniocytes by fluorescence in situ hybridization (fish), we used a contig of three overlapping cosmids mapped to 13q12.3. methods: the cosmid dna carrying the expected sequences of human chromosome 13 was isolated from host cells and labelled with biotin-11-dutp. the...

Abstract Microtubule targeting agents (MTAs) are indispensable medicines to treat a wide range of cancers. While evidence suggests that glioblastoma (GBM) is sensitive disruptions in microtubule (MT) functions; most MTAs do not reach the brain. To address this limitation, we developed brain-penetrant MTA, ST-401, kills patient-derived GBM culture at nanomolar concentrations, binds colchicine si...

This study investigates the effectiveness of the antinociceptive effects of diclofenac, an NSAID, on the nociceptive behavior of morphine-treated rats on formalin test.Rats were treated with morphine-containing drinking water for twenty one days, which induced morphine dependence. The antinociceptive effects of 8, 16, and 32 mg/kg doses of diclofenac were then evaluated and compared with distil...