SARS-CoV-2 infection inhibits interferon expression, while hyper-activating innate-immune signalling and expression of pro-inflammatory cytokines. proteins: Spike, M nsp6, nsp12 nsp13 inhibit IFR3-mediated Type-1-interferon defence, but hyper-activate intracellular signalling, which leads to dysfunctional cytokines, particularly IL-1β IL-6, IL-8, TNFα. Ezrin, a sub-membrane adaptor-protein, org...

Localization and tracking of individual receptors by single-molecule imaging opens unique possibilities to unravel the assembly dynamics signaling complexes in plasma membrane. For this purpose, we here developed a comprehensive workflow for analyzing receptor diffusion interaction live cells at single molecule level with up four colors. We engineered two monomeric GFP variants, which are ortho...

Abbreviation: HIV-1: Human Immunodeficiency Virus Type 1; CHI: Chronic HIV-1 Infection; PDCs: Plasmacytoid Dendritic Cells; IFN-α: Interferon-α; TLR: Toll-like Receptor; TLR7: Toll-like Receptor 7; TLR9: Toll-like receptor 9; IRF7: Interferon Regulatory Factor 7; NF-κB: Nuclear factor κB; NCs: Normal Controls; MSM: Men Who Have Sex With Man; PBMC: Peripheral Blood Mononuclear Cells; ICS: Intrac...

Patients with hairy cell leukemia (HCL) and chronic lymphocytic leukemia (CLL) were treated with recombinant interferon alpha A (rIFN-alpha A). The binding of iodinated recombinant interferon-alpha to baseline samples of peripheral blood mononuclear cells (PBMCs) from the leukemia patients was compared with clinical responsiveness to rIFN-alpha A. HCL patients (8/10) responded to rIFN-alpha A t...

Endotoxin tolerance is characterized by diminished expression of inflammatory cytokines after sequential exposure to Toll-like receptor stimuli. Many mechanisms contribute to tolerance; however, chromatin remodeling appears to be the most significant regulator. The type II interferon, IFN-γ, has been recognized as being able to reverse or abrogate the establishment of tolerance. Type I interfer...