Interferon gamma (IFN-γ) is one of the key cytokines in defining T helper 1 lymphocyte immuneresponses. In this study, the bovine IFN-γ gene was cloned from spleen tissue RNA using the reversetranscription-polymerase chain reaction (RT-PCR). IFN-γ cDNA was sub-cloned and expressed inmammalian expression plasmid (pcDNA3.1(+)) under the control of the human cytomegalovirus (CMV)promoter. The pred...

Background: Cesarean section delivery can lead to much maternal morbidity. Different cytokines have been reported to be influenced by the mode of delivery. Objective: To investigate the influence of mode of delivery on maternal, placental and cord sera of interferon gamma (IFN-γ), interleukin-4 (IL-4) and interleukin-10 (IL-10) levels. Methods: These three cytokines were measured using ELISA in...

Nowadays, Stroke is regarded as the second major cause of death and third morbidity globally. The ischemic stroke (IS) among most fatal kind strokes. Thus, due to importance this subject, article aims analyze role fibrosis markers (matrix metalloproteinase-9, tissue inhibitor matrix metalloproteinases-1), cytokines (tumor necrosis factor-α, interferon-γ, monocytic chemoattractant protein-1) in ...

Inflammatory bowel diseases are associated with dysregulated electrolyte and water transport and resultant diarrhea. Aquaporins are transmembrane proteins that function as water channels in intestinal epithelial cells. We investigated the effect of the inflammatory cytokine, interferon-γ, which is a major player in inflammatory bowel diseases, on aquaporin-1 expression in a mouse colonic epithe...

Objective(s): The effects of alpha linolenic acid (ALA) on tracheal responsiveness (TR), total protein (TP), phospholipase A2 (PLA2), immunoglobulin E (IgE), interleukin 4 (IL-4), interferon gamma (INF-γ) level and INF-γ/IL4 ratio in bronchoalveolar lavage fluid (BALF) of sensitized rats were examined.Materials and Methods: TR to methacholine and ovalbumin (OA), BALF levels of TP, PLA2 and IgE ...

the immunogenicity of γ-irradiated tachyzoites of a highly virulent strain of toxoplasma gondii (rh strain) was investigated. immunized mice survived challenge infection and displayed increased interferon gamma (inf-γ ) production. on the other hand, nonimmunized controls died after 10 days and displayed a significantly decreased lymphoproliferative response to concanavalin a (con a). no tachyz...

The administration of interferon has improved the antiviral and immunomodulatory abilities piglets, which is conductive to prevention potential diseases or delay appearance clinical symptoms. This study aimed evaluate effects from recombinant interferon-alpha (IFN-α) on daily care piglets. results were compared a Chinese herbal drug that was shown increase serum levels. In addition, routes oral...