While it is well known that individual integrins are critical mediators of cell behavior, recent work has shown that when multiple types of integrins simultaneously engage the ECM, cell functions are enhanced. However, it is not known how integrins spatially coordinate to regulate cell adhesion because no reliable method exists to segregate integrins on the cell membrane. Here, we use a microco...

Upon binding to the extracellular matrix protein, fibronectin, αV-class and α5β1 integrins trigger the recruitment of large protein assemblies and strengthen cell adhesion. Both integrin classes have been functionally specified, however their specific roles in immediate phases of cell attachment remain uncharacterized. Here, we quantify the adhesion of αV-class and/or α5β1 integrins expressing ...

Integrins represent ideal targets for molecular imaging and targeted therapy of cancer their role in has been reviewed extensively elsewhere. Except αVβ3 αVβ5, the remaining integrins were not systematically considered tested as potential therapeutic targets. In recent years, studies on integrin α6 a target are increasing, due to highly expressed several cancers, expression associated with poor...

At synapses, cell adhesion molecules (CAMs) provide the molecular framework for coordinating signaling events across the synaptic cleft. Among synaptic CAMs, the integrins, receptors for extracellular matrix proteins and counterreceptors on adjacent cells, are implicated in synapse maturation and plasticity and memory formation. However, little is known about the molecular mechanisms of integri...

Cells within the vascular wall connect their cytoskeleton to the extracellular matrix (ECM) through a family of cell surface receptors known as integrins. The ability of integrins to act as a link between the extracellular and intracellular environments allows transmission of inside-out and outside-in signals capable of modulating diverse vascular phenomena. In this review we summarize what is ...

Filopodia assemble unique integrin-adhesion complexes to sense the extracellular matrix. However, mechanisms of integrin localization and regulation in filopodia are poorly defined. Here, we observed that active integrins accumulate at tip myosin-X(MYO10)-positive while inactive uniformly distributed. RNAi depletion 10 activity modulators identified talin as principal activator filopodia. Delet...