The purpose of this research was to formulate insulin-loaded polycaprolactone (PCL) nanoparticles and evaluate structural stability the protein using fluorescence spectroscopy. size morphology were characterized dynamic light scattering (DLS) scanning electron microscopy (SEM). Fluorescence emission data revealed that insulin is most stable with multilayer adsorption at pH close its isoelectric...

Insulin Delivery In article number 2210392, Christoph E. Hagemeyer, Francesca Cavalieri, and co-workers report how biodegradable charge-switchable phytoglycogen nanoparticles with glucose-sensitive phenylboronic acid groups amine moieties can be engineered to form nanocomplexes insulin for its rapid efficient glucose-responsive delivery. Subcutaneous injection of in two distinct diabetic mouse ...

Purpose: To optimize the preparation of insulin-chitosan nanoparticles (ICNS) using response surface methodology (RSM). Methods: ICNS were formulated through ionic cross linking method. The effects of the ratio between insulin and chitosan, pH of the medium and rotation speed on insulin encapsulation efficiency (EE) were investigated. Box-Behnken experimental design coupled with response surfac...

Diabetes is a metabolic disease characterized by hyperglycemia due to impaired insulin secretion, action, or both. All patients with type 1 diabetes and many 2 require therapy achieve reasonable glycemic control. During this time, given through the subcutaneous injection route because it can be destroyed gastric acid when orally. Until now, studies have developed oral using various delivery sys...

introduction: oral delivery is the most favorable route for insulin administration. the aim of this study was to generate new chitosan coated insulin nanoliposomes and then to assess the physiological efficacy of these nanoliposomes after oral administration in diabetic rabbits. methods: nanoliposomes with negative surface charge encapsulating insulin were prepared by reverse phase evaporation ...

In this study, lipid modified polymeric nanoparticles for oral delivery of insulin was developed. Firstly, chitosan nanoparticles (CS NPs) was prepared by cross-linking of chitosan with triphosphate TPP as the core of the core-shell structured nanocarriers. Then lipid coating chitosan nanoparticles (LCS) was prepared by co-incubation of the CS NPs with EPC liposomes. The morphologies of these n...

In this study four quaternized derivatives of chitosan: trimethyl chitosan (TMC), dimethylethyl chitosan (DMEC), diethylmethyl chitosan (DEMC) and triethyl chitosan (TEC) with degree of substitution of approximately 50+/-5% were synthesized and their effect on the permeability of insulin across intestinal Caco-2 monolayers was studied and compared with chitosan both in free-soluble form and in ...

Nanoparticles are poised to have a tremendous impact on the treatment of many diseases, but their broad application is limited because currently they can only be administered by parenteral methods. Oral administration of nanoparticles is preferred but remains a challenge because transport across the intestinal epithelium is limited. We show that nanoparticles targeted to the neonatal Fc recepto...

Alginate-dextran sulfate-based particles obtained by emulsification/internal gelation technology can be considered suitable carriers for oral insulin delivery. A rational study focused on the emulsification and particle recovery steps was developed in order to reduce particles to the nanosize range while keeping insulin bioactivity. There was a decrease in size when ultrasonication was used dur...