The cell-surface receptor for hyaluronate is an integral membrane glycoprotein of Mr 85,000 (Underhill, C. B., A. L. Thurn, and B. E. Lacy, 1985, J. Biol. Chem., 260:8128-8133) that is thought to mediate many of the effects that hyaluronate has on cell behavior, such as migration, angiogenesis, and phagocytosis. To determine if the receptor is associated with the underlying cytoskeleton, Swiss ...

Although native chemical ligation (NCL) and related chemoselective ligation approaches provide an elegant method to stitch together unprotected peptides, the handling and purification of insoluble and aggregation-prone peptides and assembly intermediates create a bottleneck to routinely preparing large proteins by completely synthetic means. In this work, we introduce a new general tool, Fmoc-D...

Extraction of rat brain myelin in a buffer containing Triton X-100 yielded a soluble fraction and an insoluble residue that was enriched in cytoskeletal elements. Immunoblot analysis of the detergent-soluble fraction and the insoluble cytoskeletal residue showed that all of the tubulin and more than half of the actin were found within the cytoskeletal fraction. The distribution of myelin-specif...

The development of efficient pretreatments is widely used in biobased products aiming to add value the residue. In this study, orange bagasse investigated for cellulose extraction under moderate chemical sequential conditions. Three are evaluated: alkaline treatment, organosolv, and residue insoluble alcohol. Then, all pretreated samples bleached. results analyzed by composition, Fourier transf...

Whole bovine nuchal ligaments, or portions thereof (in the case of commercially valuable animals), were obtained from 45 animals (28 fetal and 17 postnatal) ranging in age from 110 days of gestation to 10 yr. Insoluble elastin was quantitatively prepared from the fresh ligaments by extraction with hot alkali and by a combination of multiple extractions with alkaline buffer and then repeated aut...

Stylolite networks and their insoluble residues (IR) are among the major concerns and the fundamental controls on the mining and processing of sedimentary decorative stones, largely made from dolomitized limestones. They are mainly used, as natural rock blocks and polished slabs, to construct and decorate floors and walls of buildings and monuments. Representative quarries in Khur areas, east o...

An expression plasmid containing a hybrid gene encoding a protein having the primary amino acid sequence of the adenylate cyclase from Escherichia coli was constructed. When the gene was induced, the adenylate cyclase could be expressed at high levels in a cya- strain of E. coli. The majority of the enzymatic activity and protein (having a molecular weight of 95,000) induced was insoluble. Howe...

Abstract Deep eutectic solvents (DESs) are new sustainable and cheap reaction media combining the features of both ionic liquids organic solvents. They made up by association hydrogen‐bond donors acceptors, they can promote hydrolysis lignocellulosic bonds. Herein microwave‐assisted treatment waste wood flours with a DES constituted choline chloride oxalic acid to get cellulosic residue separat...

Striated fibres showing a beaded appearance in the electron microscope were prepared from the insoluble collagen residue of foetal nucleus pulposus. The assembling and stability of the beaded fibres are controlled to a significant degree by electrostatic forces. In acid solutions (pH 2-0) the organization of the structures is preserved. Analytical data on the preparations show the presence of p...