Background: Phosphodiesterase 3A (PDE3A) and phosphodiesterase 3B (PDE3B) play a critical role in the regulation of intracellular level of adenosine 3´,5´-cyclic monophosphate (cyclic AMP, cAMP) and guanosine 3´,5´-cyclic monophosphate (cyclic GMP, cGMP). Subsequently PDE3 inhibitors have shown to relax vascular and inhibit platelet aggregation in cardiovascular disease. Objectives: In th...

Cochineal is the name of both crimson or carmine dye and the cochineal insect (Dactylopius coccus), a scale insect from which the dye is derived. There are other species in the genus Dactylopius which can be used to produce cochineal extract, but they are extremely difficult to distinguish from D. coccus, even for expert taxonomists, and the latter scientific name (and the use of the term "coch...

Anticarsia gemmatalis caterpillar is controlled with synthetic insecticides, which cause environmental pollution and insect resistance, new compounds low impact, need to be investigated. Toxicity, survival, locomotion, feeding, midgut histopathology of A. caterpillars exposed lemongrass essential oil (LEO) its components (citral geranyl acetate) were The are toxic survival decreasing according ...

Ethiopia is remarkably rich in its biological, ecological and landscape diversity and is home to outstanding natural bio-resources such as a number of herbs, aromatic and medicinal plants. The present study was carried out to study the composition, distribution and economic importance of insect pests of prioritized aromatic (Mentha sp., lemon verbena, fennel, rosemary, rosadamascene, oregano, p...

The relative contribution to cytotoxicity of each of the multiple NK cell activation receptors has been difficult to assess. Using Drosophila insect cells, which express ligands of human NK cell receptors, we show that target cell lysis by resting NK cells is controlled by different receptor signals for cytolytic granule polarization and degranulation. Intercellular adhesion molecule (ICAM)-1 o...

Ethiopia is remarkably rich in its biological, ecological and landscape diversity and is home to outstanding natural bio-resources such as a number of herbs, aromatic and medicinal plants. The present study was carried out to study the composition, distribution and economic importance of insect pests of prioritized aromatic (Mentha sp., lemon verbena, fennel, rosemary, rosadamascene, oregano, p...

The antiherpetic agent (E)-5-(2-bromovinyl)-2'-deoxyuridine (BVDU) was found to be an efficient substrate for recombinant Drosophila melanogaster-deoxyribonucleoside kinase with a K(m) of 4.5 microM and a V(max) of 400 nmol/microg protein/h compared with 1.3 microM and 62.5 nmol/microg protein/h, respectively, for the natural substrate thymidine. Mammalian cytosolic thymidine kinase-1 does not ...

The baculovirus genetic expression system has been used to produce murine retinoic acid receptor (RAR) type gamma in Spodoptera frugiperda insect cells and Manduca sexta insect larvae. A hydroxyapatite binding assay revealed production levels of 300 pmol of unoccupied receptor per mg of protein in insect cells, whereas levels from infected insect larvae were found to average 100 pmol of RAR gam...

BACKGROUND Various protein expression systems, such as Escherichia coli (E. coli), Saccharomyces cerevisiae (S. cerevisiae), Pichia pastoris (P. pastoris), insect cells and mammalian cell lines, have been developed for the synthesis of G protein-coupled receptors (GPCRs) for structural studies. Recently, the crystal structures of four recombinant human GPCRs, namely β2 adrenergic receptor, aden...

This investigation examined the utility of three recombinant protein-expression systems (COS cells, insect cells and insect larvae) to cost-effectively produce biologically active human butyrylcholinesterase (BuChE). It was determined that baculovirus-infected insect cells (Sf9 and High 5) expressed 3.5- and 8.2-fold, respectively, more active enzyme than COS-7 cells. Baculovirus-infected cabba...