Accumulation of calcium in the mitochondria of rat liver parenchymal cells at 16 and 24 hours after poisoning with carbon tetrachloride is associated with an increase in amount of liver inorganic phosphate, the persistence of mitochondrial adenosine triphosphatase activity, and the formation of electron-opaque intramitochondrial masses in cells with increased calcium contents. These masses, whi...

A study of the product-inhibition patterns of carbamoyl phosphate synthetase from bovine liver is reported. Inhibition by adenosine, AMP and inorganic ions is also reported. The results are in agreement with the previously proposed model in which the order of substrate binding is ATPMg, followed by HCO(3) (-), ATPMg and NH(4) (+). The order of product release on the basis of the reported result...

In view of existing evidence that inorganic phosphate of serum is capable of passing through membranes impermeable to protein, and in view of the increasing volume of evidence that glomerular urine has the composition of an ultrafiltrate, it is difficult to believe that part at least of the inorganic phosphate of urine is not excreted through the glomeruli. In compensation dialysis experiments,...

A study of the exchange reactions between adenosine triphosphate and either radioactive inorganic phosphate, or adenosine diphosphate (l), catalyzed by propionyl carboxylase, showed that inorganic phosphate was required for adenosine triphosphate-adenosine diphosphate exchange and, conversely, adenosine diphosphate was required for adenosine triphosphateinorganic phosphate exchange. A requireme...

Protein kinase activity has been detected associated with the outer surface of guinea pig peritoneal macrophages. Macrophages incubated with [gamma-32P]ATP incorporated 32P-phosphate into cell-associated proteins. Inorganic phosphate did not compete, nor could inorganic [32P]phosphate substitute as the phosphate donor, demonstrating that transfer of phosphate from ATP to protein is direct and e...

While phosphorus in the form of inorganic or organic phosphate is critically involved in most cellular functions, high plasma levels of inorganic phosphate levels have emerged as independent risk factor for cardiac fibrosis, cardiovascular morbidity and decreased life-expectancy. While the link of high phosphate and cardiovascular disease is commonly explained by direct cellular effects of phos...

A pot experiment was carried out on hybrid drumstick PKM 1 added with inorganic and organic fertilisers. Seeds of hybrid Drumstick PKM1 were sown in the plastic pots in glass house condition. Single inorganic fertilizer, phosphate solubiliser and bio organic fertilizer as well as in different combinations were applied to the pots. Data revealed that plant height, fresh and dry biomass, moisture...

Aim: To assess the potential of acute alcohol consumption to dissolve tooth surfaces and to evaluate the difference in the dissolution potential of whisky, beer and wine. Methods: The study sample comprised 36 healthy male volunteers with mean age of 26.27 (SD-1.96) years (range 25-30 years). The study design involved randomly allocating the 36 individuals into three groups of alcohol consumpti...

Autotrophic organisms obtain phosphorus from the environment by secreting alkaline phosphatases that act on esters, resulting in inorganic phosphate that is then taken up. New work shows that the cyanobacterium Aphanizomenon ovalisporum obtains inorganic phosphate by secreting the cyanotoxin cylindrospermopsin, which induces alkaline phosphatase in other phytoplankton species.

There are a large number of assays in cellular and molecular biology that either produce or consume inorganic phosphate. Therefore, the ability to quantitatively measure these phosphorous compounds can allow the assessment of the associated enzymes. Here we describe the use of the mQuant Scanning microplate spectrophotometer to quantitate ATPase activity by measuring inorganic phosphate in solu...