نتایج جستجو برای: induced pluripotent stem cells ipsc

تعداد نتایج: 2213628  

Journal: :FEBS Journal 2021

Genetic susceptibility of type 2 diabetes and Juxtaposed with another zinc finger protein 1 (Jazf1) has been reported; however, the precise role Jazf1 in metabolic processes remains elusive. In this study, using Jazf1‐knockout (KO)‐induced pluripotent stem cells (iPSC), pancreatic beta cell line MIN6 cells, Jazf‐1 heterozygous KO (Jazf1 +/− ) mice, effect on gradual differentiation was investig...

2013
Mahendra Rao

Morizane et al. (2013) show that donor-matched differentiated derivatives of induced pluripotent stem cells (iPSC) do not cause an immune response after transplantation, whereas transplantation of HLA-mismatched iPSC derivatives to the same site clearly does. The importance of these results is discussed in this commentary as we assess how best to move forward with iPSC-based cell therapy.

2017
Go Itakura Soya Kawabata Miki Ando Yuichiro Nishiyama Keiko Sugai Masahiro Ozaki Tsuyoshi Iida Toshiki Ookubo Kota Kojima Rei Kashiwagi Kaori Yasutake Hiromitsu Nakauchi Hiroyuki Miyoshi Narihito Nagoshi Jun Kohyama Akio Iwanami Morio Matsumoto Masaya Nakamura Hideyuki Okano

Human induced pluripotent stem cells (iPSCs) are promising in regenerative medicine. However, the risks of teratoma formation and the overgrowth of the transplanted cells continue to be major hurdles that must be overcome. Here, we examined the efficacy of the inducible caspase-9 (iCaspase9) gene as a fail-safe against undesired tumorigenic transformation of iPSC-derived somatic cells. We used ...

Journal: :Acta biochimica Polonica 2015
Józef Dulak Krzysztof Szade Agata Szade Witold Nowak Alicja Józkowicz

Stem cells are self-renewing cells that can differentiate into specialized cell type(s). Pluripotent stem cells, i.e. embryonic stem cells (ESC) or induced pluripotent stem cells (iPSC) differentiate into cells of all three embryonic lineages. Multipotent stem cells, like hematopoietic stem cells (HSC), can develop into multiple specialized cells in a specific tissue. Unipotent cells differenti...

2016
Soya Kawabata Morito Takano Yuko Numasawa-Kuroiwa Go Itakura Yoshiomi Kobayashi Yuichiro Nishiyama Keiko Sugai Soraya Nishimura Hiroki Iwai Miho Isoda Shinsuke Shibata Jun Kohyama Akio Iwanami Yoshiaki Toyama Morio Matsumoto Masaya Nakamura Hideyuki Okano

Murine- and human-induced pluripotent stem cell-derived neural stem/progenitor cells (iPSC-NS/PCs) promote functional recovery following transplantation into the injured spinal cord in rodents and primates. Although remyelination of spared demyelinated axons is a critical mechanism in the regeneration of the injured spinal cord, human iPSC-NS/PCs predominantly differentiate into neurons both in...

2014
WenYin He XiangJin Kang HongZi Du Bing Song ZhenYu Lu Yuling Huang Ding Wang Xiaofang Sun Yang Yu Yong Fan Meijia Zhang

BACKGROUND Epigenetic regulation is critical for the maintenance of human pluripotent stem cells. It has been shown that pluripotent stem cells, such as embryonic stem cells and induced pluripotent stem cells, appear to have a hypermethylated status compared with differentiated cells. However, the epigenetic differences in genes that maintain stemness and regulate reprogramming between embryoni...

2016
Akira Kunitomi Shinsuke Yuasa Fumihiro Sugiyama Yuki Saito Tomohisa Seki Dai Kusumoto Shin Kashimura Makoto Takei Shugo Tohyama Hisayuki Hashimoto Toru Egashira Yoko Tanimoto Saori Mizuno Shoma Tanaka Hironobu Okuno Kazuki Yamazawa Hideo Watanabe Mayumi Oda Ruri Kaneda Yumi Matsuzaki Toshihiro Nagai Hideyuki Okano Ken-ichi Yagami Mamoru Tanaka Keiichi Fukuda

Embryonic stem cells (ESCs) are a hallmark of ideal pluripotent stem cells. Epigenetic reprogramming of induced pluripotent stem cells (iPSCs) has not been fully accomplished. iPSC generation is similar to somatic cell nuclear transfer (SCNT) in oocytes, and this procedure can be used to generate ESCs (SCNT-ESCs), which suggests the contribution of oocyte-specific constituents. Here, we show th...

Journal: :Folia biologica 2012
X Yulin L Lizhen Z Lifei F Shan L Ru H Kaimin H Huang

Ectopic expression of defined sets of genetic factors can reprogramme somatic cells to induced pluripotent stem cells (iPSCs) that closely resemble embryonic stem cells. However, the low reprogramming efficiency is a significant handicap for mechanistic studies and potential clinical application. In this study, we used human bone marro...

2010
Jiang Li Guo-Dong Gao Ti-Fei Yuan

The hypothesis is that induced pluripotent stem cells (iPSC) derived Schwann cells and/or macrophages can be transplanted into acellular nerve graft in repairing injured nervous system. The efficiency of iPSC seeded acellular nerve graft may mimic the autologous peripheral nerve graft.

2016
Liana C. Boraas Julia B. Guidry Emma T. Pineda Tabassum Ahsan Xiuchun (Cindy) Tian

Many emerging cell-based therapies are based on pluripotent stem cells, though complete understanding of the properties of these cells is lacking. In these cells, much is still unknown about the cytoskeletal network, which governs the mechanoresponse. The objective of this study was to determine the cytoskeletal state in undifferentiated pluripotent stem cells and remodeling with differentiatio...

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