Morphologic determination of residual plasma cell myeloma is often difficult. This study was conducted to evaluate the diagnostic value of several ancillary methods. A total of 121 bone marrow specimens from patients with myeloma after bone marrow transplantation or chemotherapy were studied. Of those, 49 cases were studied by conventional cytogenetics (CC), 86 by flow cytometry (FC), 50 by imm...

background: fine-needle aspiration of breast cancer often provides moderate cellular material that is representative of the tumors. these samples can be used not only for cytological diagnosis but also to obtain information on the prognosis and likely response to therapy by using immunohistochemical staining studies. methods: we assessed the degree of correlation between prognostic biologic mar...

The aim of the present study was to evaluate the effect of hyperbaric oxygen therapy (HBO2) on the healing process of ischemic colonic anastomoses in rats. Forty Wistar rats were divided into four groups: control (Group I), control and HBO2 (Group II), ischemia (Group III), ischemia and HBO2 (Group IV). Ischemia was achieved by clamping four centimeters of the colonic arcade. On the eighth ther...

section was subsequently performed during the 36th week. intraoperative determination of a hemorrhagic mass in the left ovary and a pre-diagnosis of endometrioma necessitated wedge resection. Microscopic examination revealed chorionic villous-like structures within the hematoma, along with a few isolated trophoblast-like cells and membranous structures (Figure A−D). immunohistochemical analysis...

Background. Acne vulgaris belongs to androgen-dependent diseases where androgens cause sebaceous gland hypertrophy with a subsequent enlargement of sebum production.
 Aims. Determination the expression profile steroidogenesis-related proteins in normal skin and patients acne.
 Material methods. Immunohistochemical assay was carried out characterize TSPO, StAR, CYP11A1, 3-hydroxysteroi...

The accuracy of immunohistochemical image antigen determination is very important in reporting result immunohistochemical examination. The previous studies [5], [10] have not been succeeded to determine such accuracy. The failure is caused by the characters in the image cells vary widely in shape, size, cell type, outward color appearance, and the huddled cells. EsFuMos method that uses fuzzy i...

the focus of this study was to determine if early detection of mutant p53 accumulation may be an early indicator of tumor aggressiveness and transformation to invasive breast cancer. for this purpose, the p53 content of 100 human breast biopsies classified as ductal carcinoma (dcis), was evaluated by immunohistochemical method. all specimens were microscopically classified into histologic types...

BACKGROUND Amyloidosis refers to a heterogeneous group of disorders associated with the deposition of chemically distinct amyloid fibril proteins. Precise determination of chemical amyloid type has diagnostic, therapeutic, and prognostic relevance. Although immunohistochemical techniques are used routinely to determine the amyloid type, the results can be negative or inconclusive, so that bioch...

The aim of this work was to analyze methylation of the promoter sites of the ESR1 and PGR genes and to determine correlations with immunohistochemical expression of estrogen and progesterone receptors in ductal and lobular breast cancers. An observational, descriptive, molecular study was conducted on 20 ductal and 20 lobular breast cancer samples with immunohistochemical determination of estro...

Background: Amyloidosis refers to a heterogeneous group of disorders associated with the deposition of chemically distinct amyloid fibril proteins. Precise determination of chemical amyloid type has diagnostic, therapeutic, and prognostic relevance. Although immunohistochemical techniques are used routinely to determine the amyloid type, the results can be negative or inconclusive, so that bioc...