Cervical cancer is still become main health problem especially in development country. This the second most common Indonesian women. It commonly caused by infection of Human Papillomavirus (HPV) type 16 and 18. Actually if cervical was detected earlier, it shows better respond therapy than other cancers, but this opportunity not supported appropiate screening methods. Nowadays, pap smear IVA ar...

Current trends in the development of on-site diagnostic tests using immunodetection principles are proceeding in two directions: toward the immunochromatography test strip and toward the immunosensor. To develop both applications for the detection of digoxin, we prepared multilabeled antibody conjugates using colloidal gold and ferrocene. Prior to applying the digoxin antibody in immunochromato...

The aim of this study was to make the first report on canine heartworm disease in the state of Rondônia and confirm its transmission in this state. Blood samples were randomly collected from 727 dogs in the city of Porto Velho. The samples were analyzed to search for microfilariae and circulating antigens, using three different techniques: optical microscopy on thick blood smears stained with G...

PURPOSE There is a need for new technologies to study tissue-based biomarkers. The current gold standard, immunohistochemistry, is compromised by variability in tissue processing and observer bias. Reverse transcription-PCR (RT-PCR), immunocytochemistry, and reverse-phase lysate microarrays (RPM) are promising alternative technologies but have not yet been validated, or correlated, on the same ...

We developed an immunochromatography-based assay for detecting antibodies against recombinant α-galactosidase A proteins in serum. The evaluation of 29 serum samples from Fabry patients, who had received enzyme replacement therapy with agalsidase alpha and/or agalsidase beta, was performed by means of this assay method, and the results clearly revealed that the patients exhibited the same level...

The usefulness of the measurement of urinary lactoferrin (LF) released from polymorphonuclear leukocytes and of an immunochromatography test strip devised for measuring urinary LF for the simple and rapid diagnosis of urinary tract infections (UTI) was evaluated. Urine specimens were collected from apparently healthy persons and patients diagnosed as suffering from UTI. In the preliminary study...

Avian antibody technology has been used in diagnosis and therapy. A”lab-on-a-chip” system for detecting morphine in an oral fluid sample was described. An antibody against morphine was obtained in an avian system. The immunogen (M-C6-HS-BSA) was injected into Leghorn chicken. Furthermore, this antibody could be used in developing an enzyme immunoassay and immunochromatography to detect morphine...