This study aimed to examine whether a single ingestion of arginine activates the mammalian target rapamycin complex 1 (mTORC1) in rat fast- and slow-twitch skeletal muscles. In first experiment, rats were orally administered (3 or 10 mmol/kg body weight) water. The plantaris, gastrocnemius, soleus muscles excised h after administration. Immunoblot analysis showed that administration with higher...

We monitored the antibody responses of 55 treated patients with early Lyme disease and physician-documented erythema migrans. Six sequential serum samples were obtained from patients before, during, and until one year after antibiotic therapy and analyzed by in-house enzyme-linked immunosorbent (ELISA) and immunoblot assays. An immunoblot procedure utilizing a gradient gel and an image analysis...

The development of the immunoblot to detect and characterize a protein with an antisera, even in a crude mixture, was a breakthrough with wide-ranging and unpredictable applications across physiology and medicine. Initially, this technique was viewed as a tool for qualitative, not quantitative, analyses of proteins because of the high number of variables between sample preparation and detection...

Coagulase negative staphylococci have become prevalent nosocomial pathogens following the implantation of foreign bodies such as prosthetic valves, cerebrospinal fluid shunts, joint prostheses and central venous catheters. They are also the most common contaminants of blood cultures so that a means of differentiating contamination from a true bacteraemia is important.' Assessment of the clinica...

We have developed a quantitative immunoblot method to measure the mole fraction of phospholamban (PLB) phosphorylated at Ser16 (X(p)) in biological samples. In cardiomyocytes, PLB phosphorylation activates the sarcoplasmic reticulum calcium ATPase (SERCA), which reduces cytoplasmic Ca(2+) to relax the heart during diastole. Unphosphorylated PLB (uPLB) inhibits SERCA at low [Ca(2+)] but phosphor...