The humoral immune responses of NIH Swiss and SWR/J mice immunized with formalin-inactivated AKR or Gross murine leukemia virus, respectively, were examined. Both immune sera had high titers of antibodies detectable in radioimmune precipitation assays using [3H]leucine-labeled AKR virus and in radioimmunoassays using purified virion components. The predominant antibody titers were directed agai...

A differential serological screen of a lambda gt11 cDNA expression library of Plasmodium falciparum was performed in an attempt to identify novel and putative host-protective antigens of the parasite. The screening was done with two categories of sera: (i) acute-phase sera obtained from smear-positive acutely infected P. falciparum patients from various regions in India and (ii) immune sera tak...

The epithelium of the mouse lens stains intensely with antisera to glial fibrillary acidic protein (GFAP). A protein co-migrating with GFAP and immunoreactive with antisera to GFAP can be demonstrated in lens epithelium protein extracts by immunoblots. GFAP has previously been considered unique to cells of neural origin, but this study demonstrates that ectodermally derived cells express GFAP o...

The generation of polyclonal antibodies to an antigen of interest is an important technique applicable to many areas of biological research. In this chapter, we describe a basic immunization procedure designed to generate polyclonal antisera in rabbits and two methods that are commonly employed in the subsequent preliminary characterization of antipeptide antibodies raised in this way.

The serology of 70 strains of Vibrio vulnificus was studied. Seven O-antigenic groups were defined among them and it was shown that the H antigens of all the strains were identical. A heat-labile masked antigen, which inhibits O agglutination, was found in some strains. As all O antisera for V. vulnificus contained some R antibody, all diagnostic O sera must be absorbed with R organisms before ...

Variations in the ability of homogeneous antibodies to absorb group a allotype antisera suggest that these V(H) region allotypes comprise a spectrum of specificities. While no homogeneous antibody tested would completely absorb antisera to the group a specificities, the total IgG preparations from individual rabbits was capable of doing so, including preimmune IgG from the rabbits that produced...

Incorporating species-specific anti-immunoglobulin G (IgG) serum into the hemagglutination-inhibition (HI) test for influenza markedly increased sensitivity without loss of specificity. The effectiveness of anti-IgG serum for augmenting antibody titers may be influenced by the variable potency of commercial anti-IgG preparations. Maximal enhancement of HI titers was achieved when anti-IgG serum...