نتایج جستجو برای: immobilized fructosyltransferase

تعداد نتایج: 17942  

2003
N. Benkeblia S. Onodera N. Shiomi

About 80% of onion bulb dry matter is non-structural carbohydrates (Darbyshire & Henry, 1981). These carbohydrates are predominantly glucose, fructose, sucrose and low-molecular-weight fructans, while starch and raffinose are absent (Darbyshire & Henry, 1981; Benkeblia et al., 2002). The metabolism of sugars is closely linked to the dormancy and sprouting state (Kato, 1966), and the most import...

Journal: :Infection and immunity 1994
D L Kiska F L Macrina

Streptococcus mutans possesses several extracellular sucrose-metabolizing enzymes which have been implicated as important virulence factors in dental caries. This study was initiated to investigate the genetic regulation of one of these enzymes, the extracellular fructosyltransferase (Ftf). Fusions were constructed with the region upstream of the S. mutans GS5 Ftf gene (ftf) and a promoterless ...

2016
Hongbin Wei Anja Bausewein Heike Steininger Tao Su Hongbo Zhao Karsten Harms Steffen Greiner Thomas Rausch

In chicory taproot, the inulin-type fructans serve as carbohydrate reserve. Inulin metabolism is mediated by fructan active enzymes (FAZYs): sucrose:sucrose 1-fructosyltransferase (1-SST; fructan synthesis), fructan:fructan-1-fructosyltransferase (1-FFT; fructan synthesis and degradation), and fructan 1-exohydrolases (1-FEH1/2a/2b; fructan degradation). In developing taproot, fructan synthesis ...

Journal: :Applied and environmental microbiology 2001
A G Heyer R Wendenburg

We have purified a fructosyltransferase from conidia of the inulin-producing fungus Aspergillus sydowi IAM 2544 and obtained peptide sequences from proteolytic fragments of the protein. With degenerated primers, we amplified a PCR fragment that was used to screen a cDNA library. The fructosyltransferase gene from Aspergillus sydowi (EMBL accession no. AJ289046) is expressed in conidia, while no...

Journal: :biomacromolecular journal 2015
parastou rahimizadeh saeed najavand mohammad pazhang

aacel9a [β-1,4-endoglucanase, (e.c:3.2.1.4)], was immobilized onto glutaraldehyde activated chitosan macrosphere by covalent attachment. the properties of the immobilized aacel9a were investigated by determining the optimum ph and optimum temperature for activity, thermal stability, and kinetic parameters. the immobilization process shifted the enzyme’s optimum temperature from 65 °c for the fr...

Journal: :FEMS microbiology letters 2001
S A van Hijum K Bonting M J van der Maarel L Dijkhuizen

Fructosyltransferase (FTF) enzymes have been characterized from various Gram-positive bacteria, but not from Lactobacillus sp. In a screening of 182 lactobacilli for polysaccharide production only one strain, Lactobacillus reuteri strain 121, was found to produce a fructan being a levan. Here we report the first-time identification and biochemical characterization of a Lactobacillus FTF enzyme....

Journal: :biomacromolecular journal 0
tayebeh nazari department of bioscience and biotechnology, malek-ashtar university of technology, tehran, iran mahdi alijanianzadeh department of bioscience and biotechnology, malek ashtar university of technology, tehran, iran ahmad molaeirad department of bioscience and biotechnology, malek-ashtar university of technology, tehran, iran maryam khayati department of bioscience and biotechnology, malek-ashtar university of technology, tehran, iran

proteases are important enzymes that their role in various industries is undeniable. however, keeping enzymes stable during its activity in harsh conditions is so important. in this study, protease enzyme was immobilized on the porous silica particles and its stability in different temperatures and phs was evaluated. first silica particles were aminated by 3-aminopropyltriethoxysilane then the ...

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