in this paper, covalent immobilization of beta amylase enzyme on the surface of modified magnetic nano particles (znfe2o4@sio2-nh2) is reported. for doing so, at first, the magnetic nanoparticles of znfe2o4 were synthesized by chemical co-precipitation method and then tetraethyl orthosilicate (teos) and 3-aminopropyltriethoxy silane (aptes) were used for modification of znfe2o4 nanoparticles wi...

in the present study, streptomyces gulbargensis and its mutant form, s.gulbargensis mu24, immobilized on polyurethane foam were investigated for the production of l-asparaginase using groundnut cake extract as medium. the medium with an initial ph of 8.5 was inoculated with free and immobilized cells separately and then subjected to fermentation by incubation at 40oc and shaking at 200 rev/min....

Improvement of properties by immobilization http://www.scitopics.com/IMPROVEMENT_OF_ ENZYME_PROPERTIES_VIA_IMMOBILIZATION_ TECHNIQUES.html This webpage focuses on the means by which enzyme activity can be stabilized or improved in an immobilized state compared with the same enzyme free in aqueous solution. Immobilized enzymes http://www.rpi.edu/dept/chem-eng/Biotech-Environ/viet/ nelly.html Thi...

objective: organophosphorus (ops) compounds are widely used in many pesticides, insecticides and chemical nerve agents. these compounds are hazardous for humans and the environment. organophosphate hydrolase (oph) is a homodimeric protein initially isolated from pseudomonas diminuta mg and flavobacterium species. this enzyme is able to degrade a broad spectrum of toxic ops compounds. using immo...

In the present study, textural and structural characterizations of chitosan bead for immobilization of alpha amylase were studied in detail by N2 adsorption–desorption, Microspore Analysis (MP), Barrett–Joyner–Halenda (BJH) plots and Field Emission Scanning Electron Microscope (FESEM) observations. Pore structure observation revealed chemical activation of chitosan bead by glutaralde...

In this paper, covalent immobilization of beta amylase enzyme on the surface of modified magnetic nano particles (ZnFe2O4@SiO2-NH2) is reported. For doing so, at first, the magnetic nanoparticles of ZnFe2O4 were synthesized by chemical co-precipitation method and then tetraethyl orthosilicate (TEOS) and 3-aminopropyltriethoxy sil...

we expressed and purified a recombinant p. pyralis luciferase with n-terminal his-tags. the silanized ni or cu-loaded magnetic particles were prepared and used to assemble the his-tagged p. pyralis luciferase. this enzyme immobilized on functionalized magnetic nanoparticles (mnps) via electrostatic interactions of his-tag with ni2+/cu2+ ions on the surface of mnps using simple one step method. ...

In the present study, Streptomyces gulbargensis and its mutant form, S.gulbargensis mu24, immobilized on polyurethane foam were investigated for the production of L-asparaginase using groundnut cake extract as medium. The medium with an initial pH of 8.5 was inoculated with free and immobilized cells separately and then subjected to fermentation by incubation at 40oC and shaking at 200 rev/min....

Kinetic comparisons have been made between a randomly immobilized and a site-specifically immobilized subtilisin BPN' on microfiltration membranes of varying hydrophilicities in both aqueous and organic media. Site-directed mutagenesis was employed to introduce a single cysteine into the amino acid sequence of subtilisin at a location away from the active site. Immobilization of this mutant enz...