Ca2þ-activated Kþ channels, such as BK and IK channels, have been proposed to fulfill pivotal functions in neoplastic transformation, malignant progression, and brain infiltration of glioblastoma cells. Here, the ionizing radiation (IR) effect of IK Kþ channel targeting was tested in human glioblastoma cells. IK channels were inhibited pharmacologically by TRAM-34 or genetically by knockdown, c...

High-frequency firing neurons are found in numerous central systems, including the auditory brainstem, thalamus, hippocampus, and neocortex. The kinetics of high-threshold K+ currents (IK(HT)) from the Kv3 subfamily has led to the proposal that these channels offset cumulative Na+ current inactivation and stabilize tonic high-frequency firing. However, all high-frequency firing neurons, examine...

Isoegomaketone (IK) was isolated from Perilla frutescens, which has been widely used as a food in Asian cuisine, and evaluated for its biological activity. We have already confirmed that IK induced the HO-1 expression via Nrf2 activation in RAW264.7 cells. In this study, we investigated the effect of IK on the mechanism of HO-1 expression. IK upregulated HO-1 mRNA and protein expression in a do...

OBJECTIVE In chronic atrial fibrillation (cAF) the potassium current IK,ACh develops agonist-independent constitutive activity. We hypothesized that abnormal phosphorylation-dependent regulation underlies the constitutive IK,ACh activity. METHODS We used voltage-clamp technique and biochemical assays to study IK,ACh regulation in atrial appendages from 61 sinus rhythm (SR), 11 paroxysmal AF (...

exposure to mercury (hg) as a heavy metal can cause health effects. the objective of this study was to assess occupational exposure to hg in a chlor-alkali petrochemical industry in iran by determining of hg concentrations in air, blood and urine samples.the study was performed on 50 exposed subjects and 50 unexposed controls. air samples were collected in the breathing zone of exposed subjects...

A two-microelectrode voltage clamp and optical measurements of membrane potential changes at the transverse tubular system (TTS) were used to characterize delayed rectifier K currents (IK(V)) in murine muscle fibers stained with the potentiometric dye di-8-ANEPPS. In intact fibers, IK(V) displays the canonical hallmarks of K(V) channels: voltage-dependent delayed activation and decay in time. T...

IK Notes reports periodically on Indigenous Knowledge (IK) initiatives in Sub-Saharan Africa and occassionally on such initiatives outside the region. It is published by the Africa region’s Results and Learning Center as part of an evolving K partnership between the World Bank, communities, NGOs, development institutions, and multilateral organizations. The views expressed in this article are t...

We describe an algorithm that recognizes seemingly most — possibly all — intrinsically knotted (IK) graphs, and can help find knotless embeddings for graphs that are not IK. The algorithm, implemented as a Mathematica program, has already been used in [6] to greatly expand the list of known minor minimal IK graphs, and to find knotless embeddings for some graphs that had previously resisted att...

The effects of cocaine on the acetylcholine(ACh)-activated muscarinic K+ current (IK(ACh)) were assessed with the whole-cell patch-clamp technique in single atrial and left ventricular myocytes enzymatically isolated from adult ferret hearts. The density of IK(ACh) is almost 5 times greater in atrial cells than in left ventricular myocytes. Cocaine reversibly blocked IK(ACh) in a dose-dependent...