The GH gene and BMP15 gene have been used as candidate genes for ­marker-assisted selection in different livestock species. Random blood samples were obtained from 180 Zel sheep breed to study genetic polymorphism of these genes. DNA was extracted from blood samples and a 365 bp fragment from the exon V of the ovine growth hormone gene and a 312 bp region from exon II of BMP15 gene were amplifi...

Lead is one of the persistent agents found extensively in the environment and has destructive impacts on the tissues. Regarding the high spread of lead, particularly in Iran, we investigated the effects of lead acetate II on the expression level of osteocalcin gene and histological changes of bone. 40 chickens were randomly divided into four groups. As well as the libitum, the control group was...

background: antibiotic resistance and the need for long-term treatments especially for chronic infections necessitate the developmentof a vaccine against pseudomonas aeruginosa infection.objectives: in this study, recombinant exotoxin a (domains i and ii), (exoa i-ii) protein was expressed, purifid and its immunologicalcharacteristics were evaluated in a mouse model.materials and methods: the g...

Background: Lead is one of the sustainable metals with devastating effects on many tissues. This study, examined the adverse effect of lead poisoning on the gene expression of collagen type II and osteocalcin by mesenchymal stem cells (MSCs) cultured in chondrogenic and osteogenic media, respectively. Methods: We used 18 male Wistar rats, divided in 3 groups. In addition to libitum feed as the...

BACKGROUND: The H9N2 subtype of avian influenzaviruses (AIVs) have spread in Asia and Middle East countriesand have become a serious threat to poultry industry in Iran.OBJECTIVES:Characterization of genes of H9N2 subtype involvingin pathogenicity and diagnosis are crucial in control of avianinfluenza outbreaks. The Nonstructural (NS) gene and its proteinproducts (NS1 & NS2) are important as dia...

spinal muscular atrophy (sma) is an autosomal recessive neuromuscular disorder characterized by degeneration of spinal cord anterior horn cells, leading to muscular atrophy. sma is clinically classified into three sub-groups based on the age of onset and severity. the majority of patients with sma have homozygous deletions of exons 7 and 8 of the survival motor neuron (smn) gene. the purpose of ...