The venom of an Australian elapid snake, the common death adder (Acanthophis antarcticus), was chromatographed on a CM-cellulose CM52 column. One of the neurotoxic components, Acanthophis antarcticus b (toxin Aa b) was isolated in about 9.4% (A280) yield. The complete amino acid sequence of toxin Aa b was elucidated. Toxin Aa b is composed of 73 amino acid residues, with ten half-cystine residu...

This study describes the development of a new sensor with applicability in determination and quantification yjr essential amino acid (AA) L-tryptophan (L-TRP) from pharmaceutical products. The proposed is based on carbon screen-printed electrode (SPCE) modified conductor polymer polypyrrole (PPy) doped potassium hexacyanoferrate (II) (FeCN). For modification SPCE PPy FeCN, chronoamperometry (CA...

We studied the effect of various amino acid mixtures on nasal allergy induced by the intranasal application of toluene diisocyanate (TDI) in mice. In Experiment 1 (Exp. 1), mice were fed a 25% casein, soy protein isolate (SPI), egg white protein (EW) or gluten diet. In Experiment 2 (Exp. 2), mice were fed a 25% amino acid mixture diets patterned after casein (AA-casein), SPI (AA-SPI), EW protei...

amino acid dehydrogenases (l-amino acid: oxidoreductase deaminating ec 1.4.1.x) are members of the wider superfamily of oxidoreductases that catalyze the reversible oxidative deamination of an amino acid to its keto acid and ammonia with the concomitant reduction of either nad+, nadp+ or fad. these enzymes have been received much attention as biocatalysts for use in biosensors or diagnostic kit...

The amino acid requirements of Neocallimastix sp N 13 isolated from sheep rumen were studied using supplements containing either ten essential amino acids (10 EAA) or eight nonessential amino acids (8 NEAA), or both (18 AA). Although the fungus could grow in a medium containing ammonium sulphate as the sole source of nitrogen, amino acid supplements, especially 18 AA, greatly stimulated its gro...

Previous results indicated that performance of chicks fed a high level (10%) of poultry by-product meal(PBM) was lower than performance of chicks fed a corn-soybean meal (SBM) diet. The latter difference was hypothesized to be due to variation in digestible amino acid (AA) levels among the diets. This study evaluated diets containing PBM formulated on equivalent total AA basis vs. an equivalent...

Amyloid protein AA is the presumptive fragment of an acute phase serum apolipoprotein, apoSAA. Two major murine apoSAA isotypes (apoSAA1 and apoSAA2) have been identified. The NH2-terminal amino acid sequences of purified murine apoSAA1 and apoSAA2 have been examined and compared with that of murine amyloid protein AA. Our results indicate that apoSAA1 and apoSAA2 are separate gene products and...

Maternal high-protein supplements designed to increase birth weight have not been successful. We recently showed that maternal amino acid infusion into pregnant sheep resulted in competitive inhibition of amino acid transport across the placenta and did not increase fetal protein accretion rates. To bypass placental transport, singleton fetal sheep were intravenously infused with an amino acid ...

Sequencing double-stranded DNA templates has become an efficient procedure (1) being much less time-consuming than the preparation and subsequent sequencing of inserts in phage singlestranded DNA. The ability to use highly degenerate oligonucleotides instead of sequence-specific primers would make this method even more powerful for the rapid sequencing ofDNA that codes for a protein for which a...