Background: Dengue is an acute viral infection has emerged as a notable public health problem. Rapid immunochromatographic test and IgM/IgG ELISA been the mainstay of diagnosis. Combination NS1 antigen detection along with antibody increases diagnostic rates. Thrombocytopenia begins during febrile phase. Therefore, we tried to evaluate association platelet counts against (NS1 IgM/IgG) IgM in de...

CONTEXT Validation of an accurate and less cumbersome noninvasive method to detect current Helicobacter pylori infection is a requisite for any laboratory. OBJECTIVES The purpose of this study is to corroborate the usefulness of two commercially available kits NovaTec ELISA-A and ELISA-G, in the detection of ongoing H.pylori infection. MATERIALS AND METHODS Two hundred and twenty eight cons...

Nephropathia epidemica (NE), Puumala (PUU) virus infection, is a febrile disease which is commonly associated with acute renal impairment. To differentiate NE from other acute febrile illnesses, a rapid and reliable serological diagnosis is important, and a number of different protocols have recently been introduced. In the present report we describe a comparative evaluation of six PUU virus im...

Canine distemper morbillivirus (CDV) infection causes a frequently fatal systemic disease in a broad range of carnivore species, including domestic dogs. In CDV infection, classical serology provides data of diagnostic and prognostic values (kinetics of seroconversion) and is also used to predict the optimal vaccination age of pups. Routine CDV serology is still based on time- and cost-intensiv...

BACKGROUND Accuracy of rapid diagnostic tests for dengue infection has been repeatedly estimated by comparing those tests with reference assays. We hypothesized that those estimates might be inaccurate if the accuracy of the reference assays is not perfect. Here, we investigated this using statistical modeling. METHODS/PRINCIPAL FINDINGS Data from a cohort study of 549 patients suspected of d...

We have previously identified the Legionella 19-kDa peptidoglycan-associated lipoprotein (PAL) as a species-common immunodominant antigen. We describe here for the first time the excretion and detection of the PAL antigen in infected urine specimens, which is useful for the diagnosis of Legionnaires' disease. Rabbit anti-PAL immunoglobulin G (IgG) antibody was produced by immunization with the ...

A sensitive and reproducible enzyme linked immunosorbent assay (ELISA) has been developed for the detection of ricin. The assay was developed using mouse polyclonal anti-ricin antibodies produced at AMRL and commercially available antibodies. The most efficient ELISA, using a goat anti-ricin antibody as the capture antibody, a rabbit anti-ricin as the second antibody and an alkaline phosphatase...

An assay for very sensitive antigen detection is described which takes advantage of the self- assembly capabilities of semi-synthetic conjugates of DNA and proteins. The general scheme of this assay is similar to a two-sided (sandwich) enzyme-linked immunoassay (ELISA); however, covalent single-stranded DNA-streptavidin (STV) conjugates, capable of hybridizing to complementary surface-bound DNA...