A competitive internal control (IC) adapted to RT-PCR in-house assay was developed for HCV RNA detection in human pooled plasma. Also, it was applied in a multiplex RT-PCR for the HIV-1 and HCV RNA screening in human pooled plasma and plasma-derived products. A 258-bp PCR product from the 5 ́noncoding region of HCV genome was obtained. A competitive IC template was constructed by inserting a 52-...

This study was carried out in bean seeds Isparta and Antalya provinces to determine Bean common mosaic virus (BCMV) 2016. For this purpose; 106 seed samples were collected from the research area. Seed used for biological, serological, molecular methods. The incidence, as a percentage of BCMV, found 87.73% by DAS-ELISA 90.56% Immunocapture Reverse Transcriptase-Polymerase Chain Reaction (IC-RT-P...

Background and Aim: Infectious coryza (IC) or snot, is caused by Avibacterium paragallinarum leads to upper respiratory disease in poultry. Various diagnostic methods are available, including isolation identification through bacterial culture biochemical tests. However, the subsequent of A. challenging because bacteria fastidious require specific growth factors. This study aimed detect clinical...

We describe a diagnostic PCR assay (D-PCR) and a quantitative PCR assay (Q-PCR) for the detection of human cytomegalovirus (CMV) in plasma and serum. In the D-PCR, DNA was purified from plasma or serum together with internal control (IC) DNA, which monitored both DNA extraction efficiency and PCR efficiency. DNA was subjected to PCR with a single primer pair, and the amount of PCR products was ...

Equine protozoal myeloencephalitis is a progressive neurologic disease of horses most commonly caused by infection with the apicomplexan parasite Sarcocystis neurona. Factors affecting neuroinvasion and neurovirulence have not been determined. We investigated the pathogenesis of infection with S. neurona in horses with severe combined immune deficiency (SCID). Two immunocompetent (IC) Arabian h...

Members of the tumor necrosis factor (TNF) superfamily have been revealed to be associated with painful bladder syndrome/interstitial cystitis (PBS/IC). TNF ligand-related molecule 1A (TL1A) and its receptor, death receptor 3 (DR3), belong to the TNF superfamily and have been implicated in chronic inflammatory diseases. Bladder biopsies from 8 female patients clinically diagnosed with PBS/IC ac...

introduction to evaluate the expression patterns of cytokeratin (k) 12, 13, and 19 in normal epithelium of the human ocular surface to determine whether k13 could be used as a marker for conjunctival epithelium. methods: total rna was isolated from the human conjunctiva and central cornea. those transcripts that had threefolds or higher expression levels in the conjunctiva than the cornea were ...

BACKGROUND AND PURPOSE Invasive candidiasis (IC) is a significant cause of morbidity and mortality in patients with hematologic disorders and bone marrow transplant recipients. Rapid, specific and sensitive test for the timely accuracy in immunocompromised patients to reduce mortality rates and prevent IC progress is necessary. We established a real-time PCR assay on blood for the diagnosis and...

The study recorded for the first time in Egypt presence of tomato spotted wilt virus (TSWV) on cucurbits, which was confirmed using several serological tests, like double antibody sandwich ELISA (DAS-ELISA); Dot-blot assay and Immuno capture polymerase chain reaction (IC-PCR).  By Dot blot immunoassay test against TSWV antisera, ability thrips species Thrips tabaci (Lind), palmi (Karny), Frankl...