Purpose: To investigate the role of dexmedetomidine in gastric ischemia/reperfusion injury using mucosal epithelial cell (GES-1) model.
 Methods: GES-1 were subjected to oxygen-glucose deprivation conditions, followed by increasing concentrations (0.5, 1.0, or 1.5 μM) for 4 h reoxygenation. Cell viability and apoptosis determined 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bro...

Hypoxia inducible factor-1α facilitates cellular adaptation to hypoxic conditions. Hence its tight regulation is crucial in hypoxia related diseases such as cerebral ischemia. Changes in hypoxia inducible factor-1α expression upon cerebral ischemia influence the expression of its downstream genes which eventually determines the extent of cellular damage. MicroRNAs are endogenous regulators of g...

Blood vessels function as conduits for the delivery of O(2) and nutrients. Hypoxia-inducible factor 1 (HIF-1) mediates adaptive transcriptional responses to hypoxia/ischemia that include expression of angiogenic cytokines/growth factors by hypoxic cells and expression of cognate receptors for these ligands by vascular cells and their progenitors. Impairment of HIF-1-dependent responses to hypox...

Recovery from hypoxia has been shown to prolong cardiac muscle contraction, particularly the relaxation phase. The present studies were designed to examine whether incomplete relaxation between beats can result from this prolongation of contraction and relaxation in isolated muscle after hypoxia and in the canine heart after both hypoxia and acute ischemia. The relationship between heart rate a...

INTRODUCTION Hypoxia-ischemia (HI) is a major perinatal problem that results in severe damage to the brain impairing the normal development of the auditory system. The purpose of the present study is to study the effect of perinatal asphyxia on the auditory pathway by recording auditory brain responses in a novel animal experimentation model in newborn piglets. METHOD Hypoxia-ischemia was ind...

Basic fibroblast growth factor (bFGF) is a polypeptide that promotes neuronal survival and blocks excitatory amino acid (EAA) neurotoxicity in vitro at very low concentrations. In the present study, we examined whether systemically administered bFGF could prevent neuronal damage induced by either EAAs or hypoxia-ischemia in vivo. Neuroprotective effects were examined in a neonatal model of hypo...

BACKGROUND AND PURPOSE Telomerase reverse transcriptase (TERT) is tightly related to the resistance of cells to stress and injury. However, little is known about the roles of TERT in the nervous system. We try to investigate the effects of TERT on the function of astrocytes in developing rat brains subjected to hypoxia-ischemia. METHODS TERT expression was detected in rat brains with hypoxia-...

Ischemia and reperfusion activate cardiac myocyte apoptosis, which may be an important feature in the progression of ischemic heart disease. The relative contributions of ischemia and reperfusion to apoptotic signal transduction have not been established. We report here that severe chronic hypoxia alone does not cause apoptosis of cardiac myocytes in culture. When rapidly contracting cardiac my...

Perinatal hypoxia-ischemia in the preterm neonate commonly results in white matter injury for which there is no specific therapy. The subventricular zone (SVZ) of the brain harbors neural stem cells and more committed progenitors including oligodendroglial progenitor cells that might serve as replacement cells for treating white matter injury. Data from rodent models suggest limited replacement...

Electron paramagnetic resonance (EPR) spectroscopy was used to record the content of nitric oxide (NO) and copper in brain tissues (frontal lobes hippocampus) liver healthy rats after ischemia modeling. Ischemia simulated by ligation carotid arteries, followed taking 3 ml blood from common artery. Signals triple complexes (DETC) were recorded EPR (DETC)2-Fe2+-NO Cu(DETC)2. Based on direct measu...