in order to determine outcome of pregnancy with lui in patients with unexplained recurrent miscarriage whose husbands have low hypo-osmotic swelling test scores, a prospective clinical intervention was performed at a university referral clinic of recurrent abortion. out of 56 patients whose husbands had abnormal hypo-osmotic swelling tests, 43 patients underwent lui, from which only 14 patients...

The aim of this study was to evaluate the effects of vitamin E associated with rapid thawing on cryopreserved goat semen. Two bucks were used and eight ejaculates per animal were collected using artificial vagina. Semen was diluted with the following treatments: BIOXCELL (control), BIOXCELL + Equex (sodium lauryl sulphate) and BIOXCELL + vitamin E 100 μM. Semen was packaged into 0.25 mL straws ...

Cell swelling has been shown to cause activation of a variety of cardiac sarcolemmal ionic conductances including potassium channels. The aim of this study was to investigate the effect of swelling on the two subtypes of delayed rectifier potassium current (IKr and IKs) in single guinea pig myocytes using the whole-cell configuration of the patch clamp technique. When the holding potential was ...

BACKGROUND Retinal degeneration in transgenic rats that express a mutant cilia gene polycystin-2 (CMV-PKD2(1/703)HA) is characterized by initial photoreceptor degeneration and glial activation, followed by vasoregression and neuronal degeneration (Feng et al., 2009, PLoS One 4: e7328). It is unknown whether glial activation contributes to neurovascular degeneration after photoreceptor degenerat...

Müller cells are mainly involved in controlling extracellular homeostasis in the retina, where intense neural activity alters ion concentrations and osmotic gradients, thus favoring cell swelling. This increase in cell volume is followed by a regulatory volume decrease response (RVD), which is known to be partially mediated by the activation of K(+) and anion channels. However, the precise mech...

Insulin is present in the seminal plasma and involved sperm activities like motility capacitation. However, effects of insulin on viability cooled ram are not fully understood. Therefore, objective current study was to evaluate effect addition maintained at 5ºC. Sperm samples were collected from six healthy, mature Santa Inês rams. The ejaculates divided into two aliquots with (insulin group) o...