The aim of this study was to determine whether or not biochemical markers can be used as surrogate measures for the mechanical quality of tissue engineered cartilage. The biochemical composition of tissue engineered cartilage constructs were altered by varying either (i) the initial cell seeding density of the scaffold (seeding density protocol) or (ii) the length of time the engineered tissue ...

BUCCINO, R. A., E. HARRIS, J. F. SPANN, JR., AND E. H. SONNENBLICK. Response of myocardial connective tissue to development of experimental hypertrophy.. Am. J. Physiol. 2 16(2) : 425-428. 1969.-Myocardial collagen concentration was determined by measuring hydroxyproline in right and left ventricles of normal. cats and those with right ventricular hypertrophy produced by chronic constriction of...

AIM To examine the protective effect of green tea extract (GT) on hepatic fibrosis in vitro and in vivo in dimethylnitrosamine (DMN)-induced rats. METHODS HSC-T6, a rat hepatic stellate cell line, was used as an in vitro assay system. Cell proliferation, collagen content, and type 1 collagen expression were examined in activated HSC-T6 cells. Collagen was determined by estimating the hydroxyp...

Objectives- Hydroxyproline is a specific amino acid of collagen which is widely used to estimate the collagen content in biological specimens. The purpose of this study was to compare the effect of the Platelet Rich Plasma (PRP) on tendon gap healing using two different scaffold made from subcutaneous fascia and polypropylene tube. Design- Experimental study Animals - 16 young adult Guinea pigs...

Background: Tendon grafting is mostly required to repair an injury to flexor tendon and due to the importance of this tendon; it needs methods to speed up the re-vitality of the tendon allograft. OBJECTIVES: The aim was to investigate the efficacy of the mesenchymal cells and platelet rich plasma on the hydroxyproline content of frozen allograft tendons after grafting in lamb. METHODS: In this ...

Sulfated polysaccharides have shown promising effects on wound healing processes along with many other biological activities. The sulfated polysaccharides extracted from two algae species habitats in Persian Gulf were studied in vivo for their effects on collagen formation and epidermal regeneration. The polysaccharides were purified from aqueous extracts of P. tetrastromatica and P. boergeseni...

OBJECTIVE The β-glucans are long-chain polymers of glucose, which comprise the fungal cell wall, stimulate cells of the innate immune system, enhance disturbed epithelization, and have antioxidant effects. Oxidative stress has been implicated in the pathogenesis of bleomycin-induced lung fibrosis and various antioxidant agents have been studied for prevention and treatment of the disease. In th...

Staining reactions characteristic of collagen and reticulin are found in pathologically altered glomeruli (1, 2), and fibrils with the periodicity of collagen fibrils have been observed occasionally in electron micrographs of severely damaged glomerular tufts. However, as far as we are aware, fibrils have not been found by light or electron microscopy in the normal glomerular tuft, except at th...

OBJECTIVE Antineoplastic agents affect the healing of intestinal anastomosis. The aim was to evaluate the effect of 5-fluorouracil (5-FU) and mitomycin on the healing of the intestinal anastomosis and their schedule of administration. MATERIAL AND METHODS Eighty-nine male albino Charles Foster rats with a mean weight of 256.57 g were divided into six groups. Group A represents the control, wh...

Collagen quantification has long been relevant to biomedical research and clinical practice to characterize tissues and determine disease states. The hydroxyproline assay, while a broadly employed method of quantifying collagen, uses perchloric acid to dissolve Ehrlich's reagent. Since perchloric acid poses occupational safety hazards and high costs, in this study, a new hydroxyproline assay wa...