introduction: the spermatogonial stem cells (sscs) form the basis of spermatogenesis. in vitro culture of germ cells is useful for the purification and increasing of sscs for transplantation. in the present study, we examined: in vitro the effects of co-culture with sertoli cells, gdnf, scf and gm-csf on efficiency of spermatogonial cells colony formation and spermatogenesis induction in the re...

multiple sclerosis is an inflammatory disease of the central nervous system in which t cells experience a second phase of activation, which ultimately leads to axonal demyelination and neurological disability. the recent advances in stem cell therapies may serve as potential treatments for neurological disorders. there are broad types of stem cells such as neural, embryonic, mesenchymal and hem...

Adult stem cells show excellent perspectives for applications in the field of regenerative medicine. In the last two decades, great strides have been made in spermatogonial stem cell research showing the potentiality of these stem cells in organ regeneration. Spermatogonial stem cells, derived from post-natal or adult mice, not only differentiate into cell types other than the spermatozoa when ...

The complex process of spermatogenesis is regulated by various factors. Studies on spermatogonial stem cells (SCCs) have provided very important tool to improve herd genetic and different field. 0.2 to 0.3 percent of total cells of seminiferous tubules is consist of spermatogonial stem cells. To investigate and biomanipulation of these cells, proliferation and viability rate of cells should be ...

spermatogonia are the male germ line stem cells whose life long expansion is needed for permanent production of spermatozoa. the present study was designed to examine the effect of hcg treatment on germ cell proliferation following stem cell transplantation in mice. spermatogonial stem cells were isolated from neonatal mice testes and characterized by alkaline phosphatase, immunoreactivity and ...

Background Infertility affects around 15% of couples, and male factors account for 50%. Cryptorchidism is one of the most common causes for azoospermia. Generation of functional spermatids from azoospermia patients is of unusual significance for treating male infertility. It has been recently reported by peers and us that human spermatogonial stem cells (SSCs) can be clearly identified and cult...

The spermatogonial stem cell initiates and maintains spermatogenesis in the testis. To perform this role, the stem cell must self replicate as well as produce daughter cells that can expand and differentiate to form spermatozoa. Despite the central importance of the spermatogonial stem cell to male reproduction, little is known about its morphological or biochemical characteristics. This result...