Follicular fluid obtained from medium or large bovine ovarian follicles inhibited ovarian luteinizing hormone/human chorionic gonadotropin sensitive adenylate cyclase in a dose-dependent manner (150'3 mg follicular fluid protein/ml). The inhibitory activity was excluded by Sephadex G-10 and was fully retained following treatment with charcoal. Fluoride-stimulated enzyme activity was not inhibit...

background: fatty acids are known to be critically important in multiple biological functions. phospholipid fatty acids of follicular fluid, an important microenvironment for the development of oocytes, may contribute to the women’s fertility and the efficacy of assisted reproduction techniques. the aim of this study was to investigate the effect of fatty acid composition of follicular fluid ph...

background: it is reasonable to think that some biochemical characteristics of follicular fluid (ff) surrounding the oocyte may play a critical role in determining the quality of oocyte and the subsequent potential needed to achieve fertilization and embryo development. objective: this study was carried out to evaluate the levels of ff homocysteine (hcy) in ivf candidate polycystic ovary syndro...

Background: To evaluate whether success of fertilization and embryo quality are more probable when follicular fluid Anti-mullerian hormone levels are higher . Materials and Methods: 62 women who underwent IVF cycle were divided into fertilized oocytes group (n=42) and non- fertilized oocytes group (n=20) . Follicular fluid Anti-mullerian hormone levels were measured in both groups. Results: Ant...

BACKGROUND During the female reproductive cycle, follicular development and corpus luteum formation crucially depend on the fast generation of new blood vessels. The importance of granulosa cells and follicular fluid in controlling this angiogenesis is still not completely understood. Vascular endothelial growth factor (VEGF) produced by granulosa cells and secreted into the follicular fluid pl...

background: the relationship between metabolism and reproduction has been always considered as an important topic in female endocrinology. it seems that leptin is one of the involved factors in infertility. leptin, in addition to regulating body weight plays an important role in regulation of endocrine, reproductive and immune systems. the aim of this stduy is to compare serum and follicular fl...

The study investigates the correlation between oocyte maturity and fertilization and a variety of hormonal parameters in follicular fluid and ovarian granulosa cells. A methodology for purification of granulosa cells from contaminating blood cells is also established. A total of 63 follicular aspirates were collected at oocyte retrieval from 30 women superovulated using the long luteinizing hor...

BACKGROUND The aim of this study was to investigate the presence of MMP-14 and MMP-2 during human ovarian follicular development using immunohistochemistry, and the activity of MMP-2 in follicular fluid using zymography. METHODS Ovarian tissue collected from the archives of the Department of Pathology was examined and medical records and histopathology were reviewed. Follicular fluids were co...

Endothelin-like immunoreactivity specific for endothelin-1 (ET-1), endothelin-2 (ET-2) or big endothelin-1 (big ET-1) was measured, using commercially available radioimmunoassay kits, in follicular fluid collected at the time of oocyte aspiration from 36 women undergoing ovulation induction by human menopausal gonadotrophin (HMG). The relationship of ET concentrations to HMG dose, peak serum oe...

In previous studies, patients with severe peri-ovarian adhesions have been found to show low pregnancy rates and a poor response to gonadotrophin stimulation during in-vitro fertilization (IVF) treatment. The purpose of this retrospective pharmacokinetic study was to assess the diffusion of exogenous human chorionic gonadotrophin (HCG) in patients with peri-ovarian adhesions by examining the co...