seed protein usually is not sensitive to environmental fluctuations; its banding pattern is very stable which advocated for cultivars identification purpose in crop. this study was planned to study effect of water deficit on protein content and protein banding pattern of wheat genotypes. in this study five wheat genotypes were planted in early november, growing season of 2008-2009. the experime...

it is not clear what factors determine divergent outcomes of infections caused by h. pylori . in the present study, the protein profiles of different strains of h. pylori, isolated from three groups of patients with ulcerative disease, non-ulcerative gastritis and cancer disease, were analyzed using 1dsds-page. the patterns of different h. pylori strains were highly divergent. about 30.76% (7 b...

copper is most toxic metal in marine organisms. characterization of protein occurring in the metabolically active tissues of muscle (mu), hepatopancreas (hp) and gills (gl) of the spiny lobster, panulirus homarus homarus on exposure to two sub-lethal doses (9.55 and 19.1 µg/l) of copper were studied for 28 days of exposure (doe). the electrophoretic pattern of muscle, hepatopancreas and gill pr...

An extreme thermophile that produces a heat-stable proteinase was isolated from hot-spring water and classified as Thermomicrobium sp. KN-22 (growth temperature, 50-83 degrees C; and optimum growth temperature, 70 degrees C). The proteinase was purified from the culture broth of this strain by fractionation with ammonium sulfate, chromatography on columns of DEAE-cellulose and CM-Sepharose CL-6...

Isoamyl alcohol oxidase (IAAOD) was purified to apparent homogeneity on SDS-PAGE from ultrafiltration (UF) concentrated sake. IAAOD was a glycoprotein, a monomeric protein with an apparent molecular mass of 73 and 87 kDa, by SDS-PAGE and gel filtration on HPLC, respectively. IAAOD showed high substrate specificity toward C5 branched-chain alkyl alcohol (isoamyl alcohol), and no activity toward ...

The vesicular monoamine transporter, which catalyses a H+/ monoamine antiport in monoaminergic vesicle membrane, is a very hydrophobic intrinsic membrane protein. After solubilization, this protein was found to have a high tendency to aggregate, as shown by SDS/PAGE, especially when samples were boiled in the classical Laemmli buffer before electrophoresis. This behavior was analysed in some de...