Careful normalization is essential for the accurate quantitation of mRNA levels in biopsy-sized tissue samples. Commonly, normalization of the target gene with an endogenous standard, mainly housekeeping genes (HKGs), is applied. However, differences in the expression levels of endogenous reference genes have been reported between different tissues and pathological states. Therefore, we were ch...

conclusions this study indicates that satureja khuzestaniea essence has inhibitory effects on the gene expression of antibiotic resistance in bla-oxa-23 that has a high mic. given that this essence has a good inhibitory effect on gene expression in mdr acinetobacter baumannii and bla-oxa-23, the results indicate that it could be used as a natural way to prevent the growth of acinetobacter bauma...

BACKGROUND AND AIM Sexual differences in the transcript levels of various genes including the hepatic isoforms of cytochrome P450 have been extensively studied. Expression of these sexual dimorphic genes have been quantified by Northern blotting, nuclear run on assays and reverse transcriptase-polymerase chain reaction (RT-PCR) methods using numerous housekeeping genes to normalize results. Ear...

Quantitative gene expression data are often normalized to the expression levels of control or so-called "housekeeping" genes. An inherent assumption in the use of housekeeping genes is that expression of the genes remains constant in the cells or tissues under investigation. Although exceptions to this assumption are well documented, housekeeping genes are of value in fully characterized system...

Three-dimensional genome organization has been increasingly recognized as an important determinant of the precise regulation gene expression in mammalian cells, yet relationship between transcriptional activity and spatial subcompartment positioning is still not fully comprehended. Here, we first utilized genome-wide Hi-C data to infer eight types (labeled A1, A2, A3, A4, B1, B2, B3, B4) mouse ...