نتایج جستجو برای: host cell protein hcp

تعداد نتایج: 2650808  

2010
Giovanni Suarez Johanna C. Sierra Michelle L. Kirtley Ashok K. Chopra

Recently, we reported that the type 6 secretion system (T6SS) of Aeromonas hydrophila SSU plays an important role in bacterial virulence in a mouse model, and immunization of animals with the T6SS effector haemolysin co-regulated protein (Hcp) protected them against lethal infections with wild-type bacteria. Additionally, we showed that the mutant bacteria deleted for the vasH gene within the T...

Laxman S Meena, Monu M

Objective(s): The periodic binding of protein expressed by Mycobacterium tuberculosis H37Rv with the host cell receptor molecules i.e. fibronectin (Fn) is gaining significance because of its adhesive properties.  The genome sequencing of M. tuberculosis H37Rv revealed that the proline-glutamic (PE) proteins contain polymorphic GC-rich repetitive sequences (PGRS) which have clinical importance i...

ژورنال: Iranian Biomedical Journal 2016
Ilghari, Dariush, Kamyab, Ahmad Reza , Karimi Arzenani, Mohsen, Khamesipour, Ali, Mamnoon, Babak, Naserpour Farivar, Taghi,

Background: Existence of bacterial host cell DNA contamination in biopharmaceutical products is a potential risk factor for patients receiving these drugs. Hence, the quantity of contamination must be controlled under the regulatory standards. Although different methods such as hybridization assays have been employed to determine DNA impurities, these methods are labor, intensive and rather exp...

2013
Yun (Kenneth) Kang James Hamzik Michael Felo Bo Qi Julia Lee Stanley Ng Gregory Liebisch Behnam Shanehsaz Nripen Singh Kris Persaud Dale L Ludwig Paul Balderes

Recent advances in mammalian cell culture processes have significantly increased product titers, but have also resulted in substantial increases in cell density and cellular debris as well as process and product related impurities. As such, with improvements in titer, corresponding improvements in downstream processing are essential. In this study we have developed an alternative antibody harve...

2013
Xuemei M. He

General Bovine serum albumin, bovine carbonic anhydrase, and conalbumin were purchased from Sigma-Aldrich. Lactoferrin was obtained from Glanbia Nutritionals, Inc. Monoclonal antibody, mAbX, was overproduced in a Chinese hamster ovary (CHO) cell culture and previously purified by column chromatographic methods. Protein fractions were analyzed by SDS-PAGE using Criterion Tris-HCl 4–20% linear gr...

Abdol Mohammad Kajbafzadeh Fardin Amidi, Karim Nayer Nia Marziyeh Agha Hoseini Mehryar Habibi Nahid Ataie nejad, Nazila Yamini, Zohreh Mazaheri,

Objective(s):Mesenchymal stem cells (MSCs) derived from Wharton’s jelly (WJ-MSCs) are now much more appealing for cell-based infertility therapy. Hence, WJ-MSCs differentiation toward germ layer cells for cell therapy purposes is currently under intensive study. Materials and Methods: MSCs were isolated from human Wharton’s jelly and treated with BMP4, retinoic acid (RA) or co-cultured on huma...

Journal: :The Journal of Cell Biology 1999
Landon L. Moore Mike Morrison Mark B. Roth

To learn more about holocentric chromosome structure and function, we generated a monoclonal antibody (mAb), 6C4, that recognizes the poleward face of mitotic chromosomes in Caenorhabditis elegans. Early in mitosis, mAb 6C4 stains dots throughout the nucleoplasm. Later in prophase, mAb 6C4 stains structures on opposing faces of chromosomes which orient towards the centrosomes at metaphase. Colo...

Journal: :jundishapur journal of microbiology 0
xiaoli xie dairy cattle research center, shandong academy of agricultural sciences, shandong, china meng han dairy cattle research center, shandong academy of agricultural sciences, shandong, china liang zhang dairy cattle research center, shandong academy of agricultural sciences, shandong, china laixing liu dairy cattle research center, shandong academy of agricultural sciences, shandong, china zuye gu dairy cattle research center, shandong academy of agricultural sciences, shandong, china mei yang dairy cattle research center, shandong academy of agricultural sciences, shandong, china

conclusions the data indicate that esat6-cfp10 could support the survival of m. tuberculosis in the host through altering the host immune response. it also indicates that the host may gain some level of protection from a second exposure to m. tuberculosis, as evidenced by increased proliferation of nr8383-ec. background mycobacterium tuberculosis is the major pathogen of tuberculosis, which aff...

Coronavirus Disease 2019 (COVID-19) is a viral pneumonia emerged in December 2019 in Wuhan, China. Its cause is a new virus from the coronavirus family scientifically named Coronavirus Acute Respiratory Syndrome 2 (SARS-CoV-2). In this review study, articles published in English until March 23, 2020 on new coronavirus infection were reviewed. These articles are obtained by searching in PubMed, ...

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