AIM To investigate the effect of actin microfilament on potassium current and hyposmotic membrane stretch-induced increase of potassium current in gastric antral circular myocytes of guinea pig. METHODS Whole-cell patch clamp technique was used to record potassium current in isolated gastric myocyes. RESULTS When the membrane potential was clamped at -60 mV, an actin microfilament disruptor...

An automatic pipette is a very useful equipment for any laboratory carrying out Kahn tests on a large number of sera. A number of devices were tried in a field laboratory which was functioning as a serological laboratory for the CeylonArmy Command during the years 1945 and 1946. A mechanical pipette which can easily be prepared with materials salvaged from a small workshop is described below. T...

Whole cell voltage-clamp recordings from Aplysia mechanosensory neurons obtained from the pleural ganglion were used to investigate the actions on membrane currents of the neuropeptides SCP(B) and FMRFamide. At the start of whole cell recording, SCP(B) typically evoked an inward current at a holding potential of -40 mV, due to the cAMP-mediated closure of the S-type K+ channel, whereas FMRFamid...

The effect of diabetes on sarcolemmal Na(+)-K(+) pump function is important for our understanding of heart disease associated with diabetes and design of its treatment. We induced diabetes characterized by hyperglycemia but no other major metabolic disturbances in rabbits. Ventricular myocytes isolated from diabetic rabbits and controls were voltage clamped and internally perfused with the whol...

1. We investigated the glycine-induced response in ventromedial hypothalamic (VMH) neurons freshly dissociated from 8- to 12-day-old rats using the nystatin and gramicidin perforated patch recording modes. The nystatin-formed pores in the plasma membrane are permeable for both monovalent cations and anions, whereas those formed by gramicidin are permeable only to monovalent cations. Therefore, ...