Bending of purple membranes in dependence on the pH analyzed by AFM and single molecule force spectroscopy R.-P. Baumann, M. Schranz and N. Hampp, Phys. Chem. Chem. Phys., 2010 DOI: 10.1039/b919729j Bifunctional polyacrylamide based polymers for the specific binding of hexahistidine tagged proteins on gold surfaces Lucas B. Thompson, Nathan H. Mack and Ralph G. Nuzzo, Phys. Chem. Chem. Phys., 2...

We have expressed recombinant RNase P protein from Escherichia coli in high yield. A hexahistidine sequence at the amino terminus allowed protein purification in a single step. Mass spectrometry confirmed the molecular weight of the purified protein and indicated a purity of > 95%. Protein functionality was demonstrated by reconstitution of active holoenzyme.

Site-specific fluorescent labeling of proteins in vivo remains one of the most powerful techniques for imaging complex processes in live cells. Although fluorescent proteins in many colors are useful tools for tracking expression and localization of fusion proteins in cells, these relatively large tags (>220 aa) can perturb protein folding, trafficking and function. Much smaller genetically enc...

BACKGROUND Making peptide pharmaceuticals involves challenging processes where many barriers, which include production and manufacture, need to be overcome. A non common but interesting research area is related to peptides with intracellular targets, which opens up new possibilities, allowing the modulation of processes occurring within the cell or interference with signaling pathways. However,...

Recombinant clathrin protein fragments form assemblies that template gold nanocrystals in an array across the latticed surface. The nanocrystals exhibit unusual anisotropic morphologies with long range ordering, both of which are dependent upon the presence of a hexahistidine tag on the clathrin heavy chain fragments.

The barefoot Carmelite Maria de São José Salazar, a beloved disciple and faithful follower of Saint Theresa Jesus, was the author remarkable literary production, practically all it written in Lisbon, at convent Albert. This article focuses particularly on his poetic production those poems that show similarities with John Cross.

Emotionally salient information is well attended and remembered. It has been shown that infatuated individuals have increased attention for their beloved. It is unknown whether this attention bias generalizes to information related to the beloved. Moreover, infatuated individuals report to remember trivial things about their beloved, but this has not yet been tested empirically. In two studies,...

The death of legendary and philanthropist Homoeopath Dr. Girendra Pal which occurred in Jaipur on 24th August 2022, will be mourned by all Homoeopathic fraternity his beloved patients, family friends. He practiced popularised homoeopathy Rajasthan is rightly known as Father Homoeopathy Rajasthan.

This article is about Hercules, a hero from Greek Mythology who considered to be the greatest of heroes ancient mythology most beloved and glorious throughout world. He faced anger Hera which drove him mad murder. To led purification, he was forced serve his cousin Eurystheus perform 12 labors. His efforts were journey into world emotional intelligence, knowing both himself (self-awareness) als...