Real-time RT-PCR and SYBR green I melt curve analysis of a 74 bp amplicon enabled identification of Plum pox virus strains C, EA, and W, with distinct T(m)'s associated with each strain. This test is a useful supplement to a real-time RT-PCR test described earlier that was used to distinguish PPV strains D and M. A longer fragment of 155 bp was not effective for strain identification. A simplif...

Melt generation and migration are important processes for the evolution of the Earth’s interior and impact the global convection of the mantle. While they have been the subject of numerous investigations, the typical time and lengthscales of melt transport are vastly different from global mantle convection, which determines where melt is generated. This makes it difficult to study mantle convec...

[1] We present the physical basis of and validate a new remote-sensing algorithm that utilizes reflected visible and near-infrared radiation to discriminate between dry and wet snow. When applied to the Moderate Resolution Imaging Spectroradiometer (MODIS) satellite data, our discrimination algorithm has the potential to retrieve melting regions of the ice sheet at a spatial resolution of 0.25 ...

the purpose of velocity analysis is to extract the normal moveout velocity as a function of the zero-offset travel time at selected cdp locations along the seismic line. since results of velocity analysis depend on coherency estimator, an estimator that provides a high velocity resolution is essential. even though the conventional semblance method which is the most popular coherency estimator (...

By detecting and quantitatively monitoring amplified DNA as PCR progresses, real-time PCR (quantitative or qPCR) instruments provide a robust approach for gene expression, genotyping, copy number variation, mutation screening, and methylation analysis. Data quality and reproducibility are governed by how robust a real-time PCR instrument’s thermal and optical systems are, with precise thermal c...