background: granuloca cells have a key role during estroeidogenesis. objectives: the purpose of this study was to determine the effect of number of culture medium granulosa cells on estradiol concentrations and mrna codding estrogenic and progestagenic enzyme. methods: briefly, follicles between 2 and 5 mm diameter were dissected from the ovaries of adult cows and were collected by rinsing the ...

In vitro studies on cancer cell lines still constitutes a major part of cancer research. It is very important that the researchers consider the importance of basic conditions of working with different cell lines. To the best of authors knowledge, this study is the first one to report the effect of seeding density on the growth curve of three human cancer cell lines. These cell lines include hum...

The relationship between culture density and the number of microvilli on the cell surface has been studied using BHK21 cells in established monolayer culture. The number of microvilli can be estimated quantitatively by a scanning electron-microscope technique. It can be increased by applying specific antiserum, but whether antibody is applied or not a significant number of cells regularly bear ...

Hepatoblasts have the potential to differentiate into both hepatocytes and biliary epithelial cells through a differentiation program that has not been fully elucidated. With the aim to better define the mechanism of differentiation of hepatoblasts, we isolated hepatoblasts and established new culture systems. We isolated hepatoblasts from E12.5 fetal mouse liver by using E-cadherin. The E-cadh...

In addition to biochemical, physiological and morphological analysis, molecular studies provide additional information for establishing phylogenetic relationships among different species and strains of the genus Dunaliella. In the present study, based on neighbor- joining analysis of the nuclear rDNA ITS sequence, a novel strain of the green algae Dunaliella viridis was identified from Maharlu ...

Transporters mediate the influx of nutrients and excretion of metabolites in mammalian cells, playing a key role in the regulation of metabolism. They are natural targets for cell engineering to alter metabolic characteristics. The GLUT5 fructose transporter was stably expressed in a Chinese hamster ovary cell line, allowing clones to utilize fructose in place of glucose in culture medium. Comp...