Download owing intravascular delivery, an important route of administration for many clinical applications, the s the predominant site of adenovirus serotype 5 (Ad5) sequestration, thereby posing a risk of toxicity. regard, it has recently been shown that the Ad5 capsid binds to the blood coagulation factor X (FX) e Ad5 hexon protein. This interaction mediates the majority of Ad5 liver transduc...

BACKGROUND Human adenovirus 55 (HAdV-55) has caused recent outbreaks of acute respiratory disease (ARD) among adults and military trainees. The active surveillance for HAdV infections was sparse in China, and current knowledge on the HAdV-type distributions and its molecular evolution is lacking. OBJECTIVES To acquire better understanding on the prevalence and molecular evolution of HAdV-55 s...

24 Most of an intravenous dose of species C adenovirus serotype 5 (Ad5) is 25 destroyed by liver Kupffer cells. In contrast, another species C virus, Ad6, evades these 26 cells to mediate more efficient liver gene delivery. Given that this difference in Kupffer 27 cell interaction is mediated by the hypervariable (HVR) loops of the virus hexon protein, 28 we genetically modified each of the sev...

Background: The C-terminus of pVI activates the adenovirus proteinase. pVI escorts hexon into the nucleus. Results: pVI binds tightly to DNA independent of sequence, Kd = 46 nM. pVI binds tightly to hexon, Kd = 1.1 nM. Conclusion: DNA binding of pVI is first step in the activation of adenovirus proteinase. Significance: Links pVI, hexon, viral DNA and the adenovirus proteinase in virion maturat...

The interaction of human adenovirus (HAdV)-C5 and many other adenoviruses with blood coagulation factors (e.g., human factor X, FX) involves the binding of their GLA domain to the hexon capsomers, resulting in high levels of hepatotropism and potential hepatotoxicity. In this study, we tested the possibility of preventing these undesirable effects by using a GLA-mimicking peptide as a competito...

This report describes a fatal case of cerebral edema caused by adenovirus in a previously healthy 18-month-old infant who developed skin rash, pulmonary congestion, and fever and who died 6 days later. Adenovirus hexon gene sequences were detected in brain tissue and brain tissue cultures. The virus was typed as adenovirus 5.

Studies of hybridization between fowl adenovirus type 1 (chicken embryo lethal orphan virus) DNA and human adenovirus type 2 DNA revealed two short but distinct regions which cross-hybridized under stringent conditions. One of the homologous regions was located between map positions 18.1 and 19.3 and did not correspond to any gene recognized so far. The second region mapped in the hexon gene be...

Early in infection, adenovirus travels to the nucleus as a naked capsid using the microtubule motor cytoplasmic dynein. How the dynein complex is recruited to viral cargo remains unclear. We find that cytoplasmic dynein and its associated proteins dynactin and NudE/NudEL, but not LIS1 or ZW10, colocalized with incoming, postendosomal adenovirus particles. However, in contrast to physiological c...

An intertypic recombinant adenovirus with a serotype 3-like hexon gene and a serotype 16-like fiber (99% identical to that of the prototype strain of human adenovirus 16 [HAdV-16], Ch79) was isolated in Argentina from an infant admitted to the hospital with acute respiratory disease. Consistent with the results of molecular characterization, strain Arg827/04 was designated H3-F16.

Two cases of conjunctivitis caused by adenovirus type 34 (Ad34) are reported. The isolates were identified as Ad34 by the neutralization test and the PCR-sequence method of the hexon gene but as Ad14 by PCR-restriction fragment length polymorphism analysis. The genome types of these two isolates were identical to that of Ad34a.