Rigid chromatography resins, such as controlled pore glass based adsorbents, offer the advantage of high permeability and a linear pressure-flow relationship irrespective of column diameter which improves process time and maximizes productivity. However, the rigidity and irregularly shaped nature of these resins often present challenges in achieving consistent and uniform packed beds as formati...

Peak parking experiments were conducted to study the chromatographic behavior in a RPLC system consisting of a column packed with superficially porous C(18)-particles and a mixture of methanol and water (70/30, v/v). The values of the surface diffusion coefficient and the retention equilibrium constant of a column packed with superficially porous C(18)-particles were comparable to those of col...

A RP-HPLC assay method has been developed and validated for cefotaxime. An isocratic RP-HPLC was developed on a SS Wakosil II- C8 column (250 mm ˜4.6 mm i.d., 5 μm) utilizing a mobile phase of ammonium acetate buffer (pH 6.8) and acetonitrile (85:15 v/v) with UV detection at wavelength 252 nm at the flow rate 0 .8 ml/min. The proposed method was validated for sensitivity, selectivity, linearity...

A simple, precise, rapid and accurate, binary reverse phase high performance liquid chromatographic method has been developed for the determination of curcumin, Demethoxy curcumin and Bismethoxy curcumin with short run time. Chromatographic separation was achieved by using Merck C15-(250 cm × 4.6 mm) Column with mobile phase tetrahydrofuran: 1% citric acid 35: 65 was used. The flow rate was 1.2...

HetR is an essential regulator of heterocyst development in cyanobacteria. Many mutations in HetR render Anabaena incapable of nitrogen fixation. The protein binds to a DNA palindrome upstream of hetP and other genes. We have determined the crystal structures of HetR complexed with palindromic DNA targets, 21, 23, and 29 bp at 2.50-, 3.00-, and 3.25-Å resolution, respectively. The highest-resol...

A favorable isotherm open to the right to some extent is better than a linear isotherm to give shorter column height in pressure swing adsorption(PSA). We face to a problem how favorable isotherm is actually favorable in PSA operation. The purpose of this paper is to propose how to construct such a custom made isotherm which optimizes the PSA processes. The PSA mod...

A RP-HPLC assay method has been developed and validated for cefoperazone. An isocratic RP-HPLC was developed on a SS Wakosil IIC18 column (250 mm × 4.6 mm i.d., 5 μm) using a mobile phase of phosphate buffer (pH 6.8) and methanol (3:1 v/v) with UV detection at wavelength 254 nm at the flow rate 1 ml/min. The proposed method was validated for sensitivity, selectivity, linearity, accuracy, precis...