Recently, hematoporphyrin derivative (H.D.) has been reported to be an important agent in tumor detection. By utilizing H.D. and a proper activating and viewing system, one can detect malignancy by fluorescence. Hematoporphyrin tends to accumulate in tumors (1) and its red fluorescence can be utilized in the delineation of neoplastic tissue. (2). Lipson, et. al. have demonstrated that with a si...

The tumor-localizing photosensitizer hematoporphyrin derivative (HPD) is shown to undergo a simultaneous two-photon excitation into the near-ultraviolet Soret band system upon intense laser irradiation at 750 nm, a spectral region where there is no significant HPD one-photon absorbance in aqueous solution. Subsequent to this excitation, internal conversion and vibrational relaxation occur, resu...

The cytochrome P-450 in hepatic microsomes prepared from rats pretreated with hematoporphyrin derivative was shown to be rapidly destroyed in the presence of long-wave ultraviolet light. The photocatalytic destruction of the heme-protein was dependent on both the dose of ultraviolet light and of hematoporphyrin derivative administered to the animals. The destructive reaction was accompanied by ...

Studies on two-photon absorption (2PA) photodynamic therapy (PDT) by using three water-soluble porphyrin self-assemblies consisting of ethynylene-linked conjugated bis (imidazolylporphyrin) are reviewed. 2PA cross-section values in water were obtained by an open aperture Z-scan measurement, and values were extremely large compared with those of monomeric porphyrins such as hematoporphyrin. Thes...

A nonsurgical experimental system for detection of malignant tissue in the posterior ocular segment has been developed. A choroidal amelanotic malignant melanoma in rabbits served as a tumor model. Fluorescence of an hematoporphyrin derivative which is concentrated by malignant tissue was observed and measured simultaneously, using a modified binocular indirect ophthalmoscope. Clinical evaluati...

nowadays, developements in nanotechnology have leeds an increased application of this newborn technology in cancer treatment. in this study, a stabilized micelle was developed to load hpd in order to be used in photodynamic treatment of cancer in an animal model. to construct micelles, pluronic p-105 was used and stabilized to encapsulate hpd . this process includes a 24 h polymerization in whi...

In this study, the effect of photosensitizers on lipid peroxidation in the retina was investigated. Porcine retinal homogenate was exposed to illumination from a fluorescent lamp with or without photosensitizers such as hematoporphyrin, rose bengal, and fluorescein for 60 min. The control, which was exposed to illumination without photosensitizers, did not show a significant increase in lipid p...

The impact of ambient humidity on a surface acoustic wave (SAW) propagation in the structure consisting of hematoporphyrin (Hp) film deposited on a piezoelectric lithium niobate substrate has been studied. The SAW transmission dependencies on frequency were measured using an RF network analyzer, and the variation in the SAW velocity was measured using SAW delay-line oscillator technique. The in...

A procedure involving the use of the reducing agent lithium aluminum hydride (LiAlH4) has been designed to explore the nature of the oligomer linkages in the tumor-localizing component of hematoporphyrin derivative (HPD). High-performance liquid chromatography and fast-atom bombardment mass spectrometry were used to determine the reduction products. The results are consistent with a structure w...