Exosomes are gaining a prominent role in research due to their intriguing biology and several therapeutic opportunities. However, their accurate purification from body fluids and detailed physicochemical characterization remain open issues. We isolated exosomes from serum of patients with Multiple Myeloma by four of the most popular purification methods and assessed the presence of residual con...

We have developed an analytical system to measure atmospheric 3He/4He ratios precisely by using a static vacuum operation mass spectrometer and an ultrahigh-vacuum purification line. After purification of the sample, helium was introduced into the mass spectrometer. Ion beams of 3He and 4He were measured at the same time by a double collector system. A resolving power of about 700 at the 5% lev...

Helium droplets provide the possibility to study phenomena at the very low temperatures at which quantum mechanical effects are more pronounced and fewer quantum states have significant occupation probabilities. Understanding the migration of either positive or negative charges in liquid helium is essential to comprehend charge-induced processes in molecular systems embedded in helium droplets....

At the start, 1.85K operation seemed reasonable and an all-helium four solution the only one possible. It is still evident that for 1.85K operation, an allhelium four cycle is optimum, not because of lowest capital cost, but due to less pumping power. In a hybrid helium three-helium four refrigerator, the specific power for pumping helium-three is 4.0026 I 3.016 times that of pumping helium four.

Helium has always been a bit of an outsider on the Periodic Table. Its two isotopes, helium-3 and helium-4, remain liquid down to absolute zero and have the lowest liquefaction temperature among the gases. These features can be traced to the high zero-point energy relative to their attractive potential. In two dimensions, helium4 will form a liquid and become superfluid at temperatures that dep...

In underwater diving, decompression schedules are based on compartmental models of nitrogen and helium tissue kinetics. However, these models are not based on direct measurements of nitrogen and helium kinetics. In isoflurane-anesthetized sheep, nitrogen and helium kinetics in the hind limb (n = 5) and brain (n = 5) were determined during helium-oxygen breathing and after return to nitrogen-oxy...

For the purpose of deriving the helium abundances in chemically peculiar stars, the importance of assuming a correct helium abundance has been investigated for determining the effective temperature and gravity of main sequence B-type stars, making full use of the present capability of reproducing their helium lines. Even if the flux distribution of main sequence B-type stars appears to depend o...

Helium was first solidified at the famous Kamerlingh Onnes low-temperature physics laboratories in Leiden by W. H. Keesom [1] on June 25, 1926. The initial experiments by Sir Francis Simon at Oxford University and by Keesom and their collaborators focused on the melting curve, the specific heat, and the thermal conductivity of solid helium as a test of our early understanding of solids. These m...