We evaluated the effects of acetazolamide on Na+-HCO3- cotransport in basolateral membrane vesicles isolated from the rabbit renal cortex. Na+ uptake stimulated by an imposed inward HCO3- gradient was not significantly reduced by 1.2 mM acetazolamide, indicating that acetazolamide does not directly inhibit Na+-HCO3- cotransport. 4,4'-Diisothiocyanostilbene-2,2'-disulfonate (DIDS)-sensitive Na+-...

Banizs B, Komlosi P, Bevensee MO, Schwiebert EM, Bell PD, Yoder BK. Altered pHi regulation and Na /HCO3 transporter activity in choroid plexus of cilia-defective Tg737 mutant mouse. Am J Physiol Cell Physiol 292: C1409–C1416, 2007. First published December 20, 2006; doi:10.1152/ajpcell.00408.2006.—Tg737 mice have defects in cilia assembly and develop hydrocephalus in the perinatal period of lif...

Intracellular pH is under strict control in myocardium; H+ are extruded from the cells by sodium-dependent mechanisms, mainly Na+/H+ exchanger and Na+/HCO3- symport, whereas Na+-independent Cl-/HCO3- exchanger extrudes bases on intracellular alkalinization. Hypertrophic myocardium from spontaneously hypertensive rats (SHR) exhibits increased Na+/H+ exchange activity that is accompanied by enhan...

kahman is the most beneficial river in alashtar city for agriculture and aquiculture. as hydrology processes have random nature, statistics and probability are base of analysis of these processes and time series are used for this purpose. the first step in time series analysis includes parameters variation through time. second step is to stationary data, third is normalization and forth is mode...

Ae4 (Slc4a9) belongs to the Slc4a family of Cl(-)/HCO3 (-) exchangers and Na(+)-HCO3 (-) cotransporters, but its ion transport cycle is poorly understood. In this study, we find that native Ae4 activity in mouse salivary gland acinar cells supports Na(+)-dependent Cl(-)/HCO3 (-) exchange that is comparable with that obtained upon heterologous expression of mouse Ae4 and human AE4 in CHO-K1 cell...

2/3 of the reservoir surface area was approximately covered with dense populations of Myriophyllum, Ceratophyllum and Potamogeton species. The headwaters of the reservoir were considered as hard water and had both carbonatic and non-carbonatic hardness. When they were exposed to lentic condition of the reservoir and its intensive photosynthetic activities of macrophyte communities, the bicarbon...

todays, the world development is facing serious water quality issues. since protect of water quality and ecosystem healthy is an important aspect of ecohydrology. the present research aimed to analyze the temporal trends of water quality parameters of mashin zard river station in order to planning long term management approaches. in this research, trend of some significant factors of water qual...

An electrogenic Na+/HCO3- cotransport system was identified and characterized in freshly dissociated salamander Müller (glial) cells. Under voltage-clamp, these cells generated an outward current when external HCO3- concentration [( HCO3-]o) was raised. This current was Na(+)-dependent, Cl(-)-independent, and was blocked by the stilbenes 4,4'-diisothiocyanato-stilbene-2,2'-disulfonate (DIDS) an...

Soil salinity severely affects soil ecosystem quality and crop production in semi-arid arid regions. A vast quantity of data on has been collected by research organizations the Commonwealth Independent States (CIS, formerly USSR) many other countries over last 70 years, but using them current international network (irrigation reclamation strategy) is complicated. This because CIS was expressed ...

HCO3- exit across the basolateral membrane of the kidney proximal tubule cell is mediated via an electrogenic Na+:HCO3- cotransporter. In these experiments, we have studied the effect of internal pH on the activity of the Na+:HCO3- cotransport system in basolateral membrane vesicles isolated from rabbit renal cortex. Equilibrium thermodynamics predicts that in the presence of constant intravesi...