The present study deals with the oral supplementation with (50, 100 and 150 ?g/ml) synergetic effect of potassium and magnesium chloride on the fat body glycogen, protein, total lipids and haemolymph trehalose, protein of fifth instar larvae of Bombyx mori L (Lepidoptera: Bombycidae). The observed parameters significantly increased in all the treated groups when compared with those of the corre...

-Different amylase enzymes were identified by analysis of digestive fluid and haemolymph in diapausing and nondiapausing strains of silkworm, Bombyx mori. The diapausing strain showed negligible digestive amylase activity at a pH range of 3-11, while the nondiapausing strain registered strikingly higher amylase activity at pH 9.2. Higher levels of undigested starch was found in the faecal matte...

The biochemical composition of haemolymph was studied during larval and pupal period in the mulberry silkworm, Bombyx mori. The silkworm is normally reared at 25±1°C and 70±5 % RH and 31°C and 36°C constitute temperature stress conditions for the domesticated silkworm. When the larvae and pupae were exposed to selected higher temperatures, a significant decrease in the protein levels of haemoly...

The presence of an iron-binding protein in the haemolymph of the horseshoe crab, Limulus polyphemus, was detected by gel filtration of 59Fe-labelled haemolymph. Lysis of amoebocytes did not change the amount of iron-binding protein in haemolymph samples. The protein was purified to homogeneity by ion-exchange chromatography. The molecular mass of the purified protein was estimated to be 282,000...

Analysis of zymograms with SDS-polyacrilamide gel electrophoresis containing gelatin as substrate, and performed on samples of haemolymph or fat body taken from Rhodnius prolixus inoculated or not with Enterobacter cloacae, demonstrated distinct patterns of protease activities: (i) in the haemolymph two proteases were induced in insects inoculated with bacteria; (ii) two proteases were detected...

In the present study, effort has been made to find the antimicrobial activity of haemolymph collected from freshwater crab, Paratelphusa hydrodromous. The haemolymph collected was tested for antimicrobial assay by disc diffusion method against clinical pathogens. Five bacterial species, namely, Escherichia coli, Klebsiella pneumonia, Proteus mirabilis, Pseudomonas aeruginosa, Staphylococcus aur...

In Locusta migratoria, activation of phenoloxidase in the haemolymph in response to injection of laminarin is age-dependent: being absent in fifth instar nymphs and newly emerged adults, and only becoming evident four days after the final moult. This pattern of change in phenoloxidase activation correlates with the pattern of change in the concentration of apolipophorin-III (apoLp-III) in the h...

Drosophila are tolerant of high levels of dietary salt and can provide a useful model for studies of the physiology of salt stress. The effects of NaCl- and KCl-rich diets on haemolymph ionoregulation and Malpighian tubule (MT) fluid secretion, Na(+) and K(+) secretion and transepithelial potential were examined in larval and adult Drosophila melanogaster. K(+) concentrations in the haemolymph ...

Topical supply of methoprene, a juvenile hormone analogue (JHa) caused notable morphological disturbance in insects. Topical supply of methoprene to newly emerged adult female D. cingulatus caused notable disturbance and induced a dramatic reduction in the total haemolymph protein pattern and lipophorin production in tissues like fat body, ovary and haemolymph. Total protein concentration in ha...

Variations in the concentration of calcium in the haemolymph and of total body calcium in Austropotamobius pallipes have been investigated. Haemolymph calcium concentration was independent of both body size and sex. During premoult the calcium concentration of the haemolymph rose from 11-8 ITIM/1 to 15-0 HIM/I. After the moult the calcium concentration remained high during stages A and B but de...