نتایج جستجو برای: h9
تعداد نتایج: 732 فیلتر نتایج به سال:
HIV-1 promotor insertion revealed by selective detection of chimeric provirus-host gene transcripts.
To study host gene activation by retroviral promotor insertion, a polymerase chain reaction (PCR) assay was developed. This method allows a sensitive and selective detection of chimeric provirus-host gene transcripts, hallmarks of insertional activation events, which does not rely on an induction of tumor cell growth. We analysed HIV-1 infected cells of a CD4+ T-cell line (H9), infected periphe...
objectives the aim of this was to indentify the presence of a/h9n2 virus among different high risk occupational groups, in tehran and qazvin provinces in seasonal outbreak in iran. background in the last decade h9n2 avian influenza viruses had caused outbreaks in poultry in many parts of the world. this subtype could infect other animals such as human and pig. avian h9n2 virus has acquired rece...
AIM This study was carried out to monitor avian influenza (AI) infection in wild birds in Egypt. MATERIALS AND METHODS A total of 135 wild birds were examined for the presence of H5, H7, and H9 hemagglutination inhibition antibodies. Organs and swab samples of 75 birds were screened by multiplex real-time reverse transcriptase-polymerase chain reaction (RRT-PCR) to detect AI subtypes H5, H7, ...
Recurrent outbreaks of H5, H7 and H9 avian influenza viruses in domestic poultry accompanied by their occasional transmission to humans have highlighted the public health threat posed by these viruses. Newer vaccine approaches for pandemic preparedness against these viruses are needed, given the limitations of vaccines currently approved for H5N1 viruses in terms of their production timelines a...
در این کار تحقیقاتی از متیل سالیسیلات و 1-(2-برومو اتوکسی)-2-برومو اتان به منظور تهیه پیش ماده ی 1،7-بیس(?2-متیل بنزوات)-1،4،7-تری اکسا هپتان استفاده شد. در ادامه با استفاده از بنزن-1،2-دی آمین های مختلف، در حضور پلی فسفریک اسید، گروه استری به حلقه بنزیمیدازول تبدیل شده و سیستم هتروسیکلی 2,2-(((اکسی بیس(اتان-2,1-دی ایل))بیس(اکسی))بیس(2,1-فنیلن))بیس(h1-بنزو[d]ایمیدازول) های مربوطه سنتز شد. س...
Many different culture systems have been developed for expanding human pluripotent stem cells (hESCs and hiPSCs). In general, 4-10 ng/ml of bFGF is supplemented in culture media in feeder-dependent systems regardless of feeder cell types, whereas in feeder-free systems, up to 100 ng/ml of bFGF is required for maintaining long-term culture on various substrates. The amount of bFGF required in na...
Colicin Ia is a bactericidal protein of 626 amino acid residues that kills its target cell by forming a channel in the inner membrane; it can also form voltage-dependent channels in planar lipid bilayer membranes. The channel-forming activity resides in the carboxy-terminal domain of approximately 177 residues. In the crystal structure of the water-soluble conformation, this domain consists of ...
PURPOSE MicroRNAs (miRNAs) in biological fluids are potential biomarkers for the diagnosis and assessment of urological diseases such as benign prostatic hyperplasia (BPH) and prostate cancer (PCa). The aim of the study was to identify and validate urinary cell-free miRNAs that can segregate patients with PCa from those with BPH. METHODS In total, 1,052 urine, 150 serum, and 150 prostate tiss...
The generation of cardiomyocytes from human embryonic stem cells (hESC) enables a variety of potential therapeutic and diagnostic applications. However, progress is challenged by the low efficiency of cardiomyocyte differentiation. Recently, Kattman et al., 2011 showed that individual hESC lines required proper balance of the Activin A and BMP4 signaling for efficient cardiac differentiation, p...
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