The behaviour of Escherichia coli O157:H7 was studied during the manufacture and storage of Iranian white cheese in brine. Cheese was manufactured using pasteurized cow milk and inoculated with E. coli O157:H7 with inoculum level of 103 cfu/ml. Four treatments were designed. Cheeses were made with or without starter culture and kept immersed in 6 or 8% salt brine during ripening and storage. Ch...

Background: Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is an infectious zoonotic pathogen causing human infections. These infections, in some cases, can lead to hemolytic uremic syndrome and its life-threatening complications and even death worldwide. The first intimate bacterial adhesion, intimin (I), with its own receptor translocated intimin receptor (Tir) and E. coli secreted protein...

The purpose of this study was to investigate the effect of different levels of Pseudomonas fluorescens (10(2) and 10(6) log10 cfu/ml) and Lactobacillus plantarum (10(2) and 10(4) log10 cfu/ml) on the growth of Escherichia coli O157:H7 on beef loins. Beef loins inoculated with E. coli O157:H7 and P. fluorescens were aerobically stored for 7 days at 4 ºC, while those inoculated with E. coli O157:...

Growing concern on the emergence of enterohaemorrhagic Escherichia coli 0157:H7 (EHEC) in the developed countries associated with cattle, poultry, vegetable and pond water have focused our attention on the prevalence of this disease from diarrhoeal patients in Jos, Nigeria. From February 2002 to February 2004, 1050 faecal specimens from adults and children of both sexes with diarrhoeal (850) an...

background escherichia coli o157:h7 is an enteric pathogen which can be frequently found asymptomatically in ruminant mammals, but can cause diseases from mild diarrhea to hemolytic uremic syndrome in humans. methods we developed fluorescent amplification-based specific hybridization (flash–pcr) assay to detect the stx-encoding gene stx-1 of e. coli o157:h7. result pcr product of 336 bp was suc...

background and objective: escherichia coli o157:h7, an emerging pathogen, causes severe enteritis and the extraintestinal complication of hemolytic-uremic syndrome. the goal of this study was to evaluate the conjugate of e. coli o157: h7 lipopolysaccharide (lps) with diphtheria toxoid (dt) as a candidate vaccine in mice model. material and methods: lps from e. coli o157:h7 was extracted by hot ...

Twelve ruminally cannulated cattle, adapted to forage or grain diet with or without monensin, were used to investigate the effects of diet and monensin on concentration and duration of ruminal persistence and fecal shedding of E. coli O157:H7. Cattle were ruminally inoculated with a strain of E. coli O157:H7 (10(10) CFU/animal) made resistant to nalidixic acid (Nal(r)). Ruminal and fecal sample...

A novel, fast and sensitive determination strategy for E. coli O157:H7 has been developed by combination of ligandmagnetic nanoparticles (LMNPs) enrichment with a fluorescent silica nanoparticles (FSiNPs) based two-color flow cytometry assay (LMNPs@FSiNPs-FCM). E. coli O157:H7 was first captured and enriched through the lectin concanavalin A (Con A) favored strong adhesion of E. coli O157:H7 to...

enterohemorrhagic escherichia coli (ehec), such as e. coli o157:h7, are emerging food-borne pathogens worldwide. this micro-organism can damage the epithelial tissue of the large intestine. the cytotoxic effects can be neutralized by probiotics such as bifidobacterium bifidum. probiotics are viable cells that have beneficial effects on the health of the host. the preventing activity of b. bifid...

Soil microcosms were inoculated with Escherichia coli O157:H7 to test persistence in fallow soil, on roots of cover crops and in presence of manure. In fallow soils, E. coli O157:H7 persisted for 25-41 days, on rye roots for 47-96 days and on alfalfa roots, in a silt loam soil, for 92 days whereas on other legumes persistence ranged from 25-40 days, similar to fallow soil. Manure did not seem t...