The hydrodynamic behavior of bovine myelin basic protein was studied by gel filtration through Sephadex G-100 under conditions which included variations in pH from 2 to 12, variations in ionic strength from 0.01 to 1.5 M at pH 2 and from 0.1 to 2 M at pH 7, and variations in guanidinium chloride concentration from 0 to 6 M. A number of well characterized compact globular proteins were subjected...

The use of low concentrations of urea, guanidinium chloride or arginine has been reported in the literature to increase protein refolding and yield of active proteins by suppressing aggregate formation. However, no studies have yet examined whether these substances can exert synergistic or cooperative effects when used in combination. In this work, a comparative study was carried out on refoldi...

Bovine nasal cartilage was studied by electron microscopy before and after extraction with 4 M guanidinium chloride or 1.9 M CaCl(2). These solvents removed matrix granules, basophilia, and 85% of the proteoglycan complex, measured as hexuronate. Simultaneously, many collagen fibrils were disaggregated into component microfibrils (approximately 40 A thick). In contrast to the above solvents, ex...

Small-angle neutron scattering measurements were performed on two very different proteins unfolded by guanidinium chloride. It is shown that the neutron spectra given by unfolded phosphoglycerate kinase and p-casein are similar to those of excluded volume homopolymer chains. INTRODUCTION A functional native protein generally adopts a specific three-dimensional structure that is determined by it...

The crystal structure of the new compound 3-guanidinium-2-aminopropionic acid hexafluorosilicate was studied by single X-ray diffraction at various temperature (100–400 K) and pressure conditions (10 –4 –0.75 GPa). Anisotropy both thermal expansion compressibility correlated with hydrogen bonding patterns especially stiffness small ring patterns. measured second harmonic generation response for...

1. New preparations of reduced carboxymethylated /3-ovomucin (S-carboxymethylf,-ovomucin) were homogeneous by sedimentation analysis, analytical sedimentation to equilibrium in CsCI gradients, and disc electrophoresis in sodium dodecyl sulphate. 2. Degradation of S-carboxymethyl-f/-ovomucin with either CNBr or trypsin indicated the presence of a subunit (approx. mol.wt. 112300). 3. Electron mic...

The cartilage matrix protein is a major non-collagenous protein in bovine cartilage. It was purified from a 5 M-guanidinium chloride extract of bovine tracheal cartilage by sequential CsCl-density-gradient centrifugation, gel chromatography in guanidinium chloride and differential precipitation. The molecular weight of the intact protein is 148 000, determined by sedimentation-equilibrium centr...

Many ions are known to affect the activity, stability, and structural integrity of proteins. Although this effect can be generally attributed to ion-induced changes in forces that govern protein folding, delineating the underlying mechanism of action still remains challenging because it requires assessment of all relevant interactions, such as ion-protein, ion-water, and ion-ion interactions. H...

In the crystal structure of the title compound, C(11)H(26)N(3) (+)·Cl(-)·C(4)H(4)N(4), the (2Z)-2,3-diamino-but-2-ene-dinitrile (Z-DAMN) mol-ecules are connected with the chloride ions via N-H⋯Cl hydrogen bonds, forming ribbons running along the a axis. The guanidinium ions are located in between the ribbons formed by Z-DAMN mol-ecules and chloride ions.